摘要
为研究低温环境条件下免疫应答对民猪激素水平的影响,选择健康的仔猪(民猪及大白猪),达到21日龄时,接种猪瘟兔化弱毒疫苗,达到48日龄时,检测的猪瘟抗体,根据结果选择体重、抗体水平接近的仔猪20头,大白猪仔猪20头,随机分组,分别为低温环境两组(1组、2组)及适温环境两组(3组、4组),其中1组为10头民猪,2组为10头大白猪,3组为10头民猪,4组为10头大白猪。试验期14 d,以猪瘟疫苗接种作为免疫应答手段,用ELISA方法对血清中方法检测干扰素-α(IFN-α)、白细胞介素6(IL-6)、猪瘟抗体(CSF)含量进行测定。结果表明:2组的IFN-α含量在14 d时、IL-6含量在7 d和14 d时、CSF在14 d时显著低于其他3组(P<0.05)。说明低温环境对民猪免疫应答的影响程度低于大白猪,民猪的抗冷应激能力强于大白猪。
In order to study the effect of immune response on the level of Min pig hormone in low temperature environment,clinical healthy Min piglets and Large White piglets were selected,were vaccinated with the swine plague vaccine at 21 days of age,blood samples were collected for detection of swine plague antibody levels at 48 days of age,20 Min piglets and 20 White piglets with similar antibody levels and similar body weight were used and were divided into four groups randomly:low and normal group,normal temperature group in the two groups,the first group of 10 Min pigs were raised at low temperature,the second group of 10 White pigs were feeding at low temperature,the third group of 10 Min pigs were raising in normal temperature,the fourth group of 10 white pigs were feeding in normal temperature.To declare ELISA result,Interferon-α(IFN-α),Interleukin-6(IL-6)and Swine plague antibody(CSF)were determined.The results showed that 14 days of immunization,IFN-αconcentrations of 2 group reduced than the other three groups respectively(P<0.05).IL-6 concentration significantly reduced in 7 days and 14 days immunization,CSF concentrations of 2 group was significantly reduced than the other three groups(P<0.05).The results showed that the immune response in low temperature environment had less influence on min pigs than the large white pigs,and the min pigs were more resistant to cold stress than the large white pigs.
作者
彭福刚
孙金艳
李忠秋
王文涛
吴赛辉
汪亮
刘娣
PENG Fu-gang;SUN Jin-yan;LI Zhong-qiu;WANG Wen-tao;WU Sai-hui;WANG Liang;LIU Di(Animal Husbandry Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)
出处
《黑龙江农业科学》
2019年第3期69-72,共4页
Heilongjiang Agricultural Sciences
基金
黑龙江省农业科学院院级重点资助项目(2017XQ15)
现代农业(生猪)产业技术体系建设专项资金资助项目(CARS-35)
关键词
低温
民猪
猪瘟抗体
内分泌
免疫
low temperature
Min pigs
swine fever antibody
internal secretion
immune
