摘要
本研究旨在比较牛A型多杀性巴氏杆菌高毒力株(PmCQ2)和低毒力株(PmCQ6)脂多糖(LPS)对小鼠巨噬细胞RAW264.7内TLR4信号通路的影响。选用体外培养RAW264.7细胞,经LPS刺激后检测细胞内TLR4信号激活及对TNF-α和IL-12p40表达的影响;并利用Western blot检测LPS对IκBα磷酸化及NF-κBp65活化的影响。结果显示,高毒力株LPS_(PmCQ2)和低毒力株LPS_(PmCQ6)分别刺激RAW264.7细胞后,均能显著提高TLR4的表达,并诱导细胞因子TNF-α和IL-12p40的分泌表达;LPS_(PmCQ2)和LPS_(PmCQ6)均能诱导IκBα磷酸化,促进NF-κBp65核转位,但二者没有显著差异(P>0.05)。本研究表明牛A型多杀性巴氏杆菌高、低毒力株LPS均能参与TLR4介导的小鼠巨噬细胞免疫应答,且二者对TLR4介导的IκBα-NF-κB信号通路的作用无显著差异,暗示牛A型多杀性巴氏杆菌对小鼠巨噬细胞的毒力与LPS无明显相关性,可能由其他毒力因子决定。
This study was designed to investigate the effects of TLR4 signaling pathway in RAW264.7 cells treated with lipopolysaccharide (LPS) in two different virulent Pasteurella multocida serotype A isolates (high-virulent PmCQ2 and low-virulent PmCQ6) respectively. The activation of TLR4 pathway, expression of TNF-α and IL-12p40, phosphorylation of IκBα, and nuclear translocation of NF-κB, were identified in LPS-stimulated RAW264.7 cells. Results showed that the expression of TLR4, TNF-α and IL-12p40 was significantly increased in LPS-stimulated RAW264.7 cells. Furthermore, both LPS PmCQ2 and LPS PmCQ6 up-regulated IκBα phosphorylation, and activated TLR4-triggered nuclear translocation of NF-κBp65, but there was no significant difference between the two treatments. The LPS of bovine Pasteurella multocida Serotype A high- and low-virulent strains can induce the TLR4-mediated immune response, and there is no difference in TLR4-mediated IκBα-NF-κB signaling pathway, indicating that there may be no significant difference in virulence between LPS PmCQ2 and LPS PmCQ6 on RAW264.7 cells. These results suggested that for RAW264.7 cells, the virulence of Pasteuralla multocida is dependent on others virulence factors, not LPS.
作者
李艳红
刘洁
李伟平
崔雨婷
彭远义
吴正理
LI Yanhong;LIU Jie;LI Weiping;CUI Yuting;PENG Yuanyi;WU Zhengli(College of Animal Science and Technology,Southwest University,Chongqing 400715,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第3期620-626,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31770965
31600716)
重庆市基础科学与前沿技术研究专项(cstc2017jcyjAX0420)
中央高校基本科研业务费专项资金(SWU114011
SWU114015)
国家现代农业(肉牛牦牛)产业技术体系建设专项基金(CARS-37)
