摘要
山东省桌规模化奶牛场10～30日龄的犊牛出现发热、咳嗽和关节肿大的临床症状，并造成部分犊牛死亡。为查明死亡原因并制定相应的防控方案，在流行病学调查和现场剖检的基础上，对采集的病料进行病理组织学检查、细菌分离鉴定、生化试验，并对鉴定的病原菌进行动物感染试验和药敏试验，对分离菌的PCR产物进行多杀性巴氏杆菌特异性基因和荚膜血清型特异性基因序列的测定。结果表明，该场犊牛主要死于以纤维素性肺炎为特征的败血症，死亡原N为多杀性巴氏杆菌感染所致，且分离的多杀性巴氏杆菌为英膜血清A型高致病性菌株，对小鼠呈高致死性；该分离菌株对林可霉素、头孢噻呋、多西环素耐药，对恩诺沙星、氟苯尼考高度敏感。
The calves in 10-30 days manifested fever,cough,arthroncus and death in a large scale dairy farm of Shandong province. To find out the cause of death and formulate prevention and con trol meatures, all the cases were confirmed by histopathologic examination, biochemical reaction, isolation and identification of bacteria on the basis of epidemiological survey and necropsy. After animal infection test and drug sensitive test to pathogenic bacteria,PCR product of isolated bacteria was determined by specific genes and the specific gene sequences of capsular serotype. The results indicated that the calves died from infection with Pasteurella multocida and the isolated strain belonged to capsular serotype A of Pasteurella mutocida which was fatal to mice. The isolated Pasteurella multocida strain was highly sensitive to levofloxacin,enroftoxacin and florfenicol.
作者
刘朋
陈萌
程子龙
闫振贵
刘思当
LIU Peng;CHEN Meng;CHENG Zi-long;YAN Zhen-gui;LIU Si-dang(College of Animal Science and Technology,Shandong Agricultural University,Tai＇an,Shandong 271000,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第8期1548-1552,共5页
Chinese Journal of Veterinary Science
基金
山东省牛产业技术体系资助项目(SDAIT-09-04)
关键词
犊牛
多杀性巴氏杆菌
荚膜血清A型
PCR
分离鉴定
calf
Pasteurella multocida
capsular serotype A
PCR
isolation and identification
