摘要
目的分析依达拉奉对水通道蛋白4(AQP4)、水通道蛋白9(AQP9)表达的影响,探讨依达拉奉对心搏骤停(CA)大鼠心肌保护作用机制。方法选取96只健康SD大鼠,将其采用随机数字表法分为假手术组(28例),依达拉奉组(34例)、生理盐水组(34例),依达拉奉组及生理盐水组诱导建立CA模型,模型建立5 min后开始进行心肺复苏(CPR),待自主循环恢复后,依达拉奉组立即静脉注射3 mg/mL依达拉奉,生理盐水组给予等体积生理盐水,假手术组不诱导CA模型,仅进行动、静脉置管及气管插管。连续监测三组心电图及血流动力学1 h后缝合伤口,待模型大鼠苏醒后拔除插管,放入笼中常规饲养。分别于自主循环恢复后12、24、48、72 h处死大鼠,测定三组心肌组织中超氧化物歧化酶(SOD)、丙二醛(MDA)水平,光学显微镜下观察不同时间点心肌病理学变化、测定脑组织含水量,采用实时荧光定量聚合酶链式反应(RT-PCR)检测脑组织AQP4、AQP9 mRNA水平。结果三组大鼠体质量、平均动脉压、舒张压、收缩压及心率等基础参数比较,均差异无统计学意义(P>0.05);12、24、48、72 h处死大鼠,假手术组心肌组织中SOD水平均明显高于依达拉奉组、生理盐水组,差异有统计学意义(P<0.05),MDA水平明显低于依达拉奉组及生理盐水组(P<0.05);依达拉奉组12、24、48、72 h心肌组织中SOD水平分别为[(76.54±9.03)、(66.53±8.25)、(57.21±7.82)、(67.02±8.47)U/mg]均明显高于生理盐水组(P<0.05),依达拉奉组12、24、48、72 h心肌组织中MDA水平[(11.36±1.15)、(18.29±2.96)、(23.07±2.37)、(20.83±1.82)nmol/mg]明显低于生理盐水组(P<0.05);各时间点生理盐水组心肌病理学损伤程度均高于依达拉奉组;12、24、48、72 h处死大鼠,依达拉奉组脑组织含水量[(74.36±7.41)、(74.51±7.31)、(75.32±7.25)、(74.23±7.14)%]均明显低于生理盐水组(P<0.05),依达拉奉组AQP4水平分别为[(1.21±0.11)、(1.13±0.12)、(0.87�
Objective To analyze the effects of edaravone on the expression of aquaporin 4(AQP4)and aquaporin 9(AQP9),and to explore the mechanism of edaravone on myocardium protective in rats with cardiac arrest(CA).Methods Ninety-six healthy SD rats were selected and randomly assigned into sham operation group(28 cases),edaravone group(34 cases),and saline group(34 cases).Edaravone group and saline group were induced to establish CA models,and cardiopulmonary resuscitation(CPR)was started at 5 minutes after the establishment of the model,after the recovery of autonomic circulation,the edaravone group was injected with 3mg/mL edaravone intravenously immediately,the saline group was given the same volume of saline,the sham operation group did not induce CA model,but only underwent arterial catheterization,venous catheterization,and tracheal intubation.The electrocardiogram and hemodynamics were continuously monitored in three groups,after 1 hour,the wound was sutured,and the intubation was pulled out after the model rats awakened,and the rats were put into the cage for routine feeding.The rats were sacrificed at 12,24,48,and 72 hours after the recovery of autonomic circulation,the levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in myocardium of three groups were measured,the myocardial pathological changes at different time points were observed under optical microscope,and water content in brain tissues was measured,real-time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detect the levels of AQP4 and AQP9 in brain tissues.Results There were no significant differences in basic parameters such as body mass,mean arterial pressure,diastolic pressure,systolic pressure,and heart rate among the three groups(all P>0.05);the rats were killed at 12 h,24 h,48 h,and 72 h,the level of SOD in myocardial tissues in sham-operated group was significantly higher than that in edaravone group and saline group(P<0.05),MDA level was significantly lower than that in edaravone group and saline group(P<0.05);the l
作者
李丽
LI Li(Department of Emergency,The People s Hospital of Jiaozuo City,Jiaozuo,Henan 454000,China)
出处
《安徽医药》
CAS
2019年第4期687-691,共5页
Anhui Medical and Pharmaceutical Journal
