摘要
[目的]本文旨在了解秀珍菇子实体形成的分子机制,为培育优质、高产、抗逆的秀珍菇品种提供理论基础。[方法]构建秀珍菇高质量c DNA文库,采用illumina高通量测序技术对秀珍菇菌丝体和子实体进行转录组测序,并利用生物信息学方法开展基因表达谱的研究以及功能基因的预测。[结果]共获得81 693个非重复序列基因(universal gene,Uni Gene),经BLAST比对NR数据库,可比对上的Uni Gene数量为61 306个。NR数据分析显示,秀珍菇已比对的Uni Gene与糙皮侧耳相似度最高,占总数目的 86.1%。同时,对秀珍菇转录组的Uni Gene进行了生物学通路的注释和预测,共注释14 315个Uni Gene,且部分与糖类代谢、氨基酸代谢、信号传导、翻译相关通路有关,分别为1 683、1 241、1 243和1 372个,表明富集在上述通路中的Uni Gene在秀珍菇子实体形成过程中可能起到重要作用。通过分析SNP突变,发现秀珍菇中分别有10 967和10 683个位点发生C/T转换和A/G转换,同时,有2 102个位点发生A/C颠换。通过查找分析SSR位点发现,共获得7 574个SSR位点,占Uni Gene总数的比例为9.27%。其中,单核苷酸重复在所有SSR重复类型中所占比例最高,为45.14%,其次为三核苷酸重复类型,为31.94%。[结论]通过高通量测序技术获得秀珍菇菌丝体和子实体的转录组数据,结合生物信息学分析,为了解秀珍菇群体遗传多样性、构建遗传连锁图谱和研究其不同生长阶段差异表达基因及其功能奠定基础。
[Objectives] This paper aimed to understand the molecular mechanism of fruiting body formation of Pleurotus pulmonarius and provide theoretical basis for breeding high-quality,high-yield and stress-resistant varieties of P.pulmonarius.[Methods] In this study,on the basis of building high quality cDNA library of P.pulmonarius,the transcriptome of mycelium and fruiting body of P.pulmonarius was sequenced by illumina high-throughput sequencing technology.In addition,bioinformatics methods were used to study gene expression profiles and predict functional genes.[Results] It showed that 81693 UniGenes were obtained through sequence splicing and 61306 UniGenes were annotated through NR database by BLAST comparison.By statistical species distribution,P.pulmonarius transcriptome had the most similar UniGenes to Pleurotus ostreatus.Meanwhile,a total number of 14315 UniGenes were identified by annotating and forecasting the biological pathways for the transcriptome of P.pulmonarius.Among 14315 UniGenes,parts of their biological functions were related to carbohydrate metabolism(1683),amino acid metabolism(1241),signal transduction(1243)and translation(1372).It was noted that these UniGenes enriched in afore-mentioned pathway might play a very important role in the process of fruiting body formation of P.pulmonarius.10967 C/T and 10683 A/G conversion,2102 A/C transversion were found by SNP analysis.Meanwhile,7574 SSR in 81693 UniGenes were found which took 9.27% of the total number of UniGenes.The characteristic of SSR distribution showed that mono-nucleotide repeat was the highest,which was 45.14% of the total,followed by tri-nucleotide repeat,which was 31.94%.[Conclusions] The transcription group data were obtained of the mycelium and fruiting body of P.pulmonarius by high-throughput sequencing technology.Combined bioinformatics analysis,this study laid the foundation for analysing the genetic diversity,constructing genetic linkage map,and researching the differentially expressed genes and its functions at different develo
作者
王伟科
宋吉玲
闫静
袁卫东
陆娜
WANG Weike;SONG Jiling;YAN Jing;YUAN Weidong;LU Na(Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2019年第2期292-299,共8页
Journal of Nanjing Agricultural University
基金
浙江省农业(食用菌)新品种选育重大科技专项(2016C02057)
关键词
秀珍菇
菌丝体
子实体
转录组测序
高通量测序技术
Pleurotus pulmonarius
mycelium
fruiting body
transcriptome sequencing
high-throughput sequencing technology
