摘要
建立化妆品中邻伞花烃-5-醇含量的高效液相色谱检测方法。样品用甲醇超声提取15 min,过0.22μm滤膜,以X-bridge C_(18)为色谱柱,乙腈–水(60∶40)为流动相,流量为1.0 mL/min,柱温为30℃,检测波长为280 nm,采用二极管阵列检测器检测。邻伞花烃-5-醇的质量浓度在1.0～100.0 mg/L范围内与色谱峰面积呈良好的线性关系,相关系数为0.999 9,检出限为3.0 mg/kg,定量限为10.0 mg/kg。样品加标回收率为93.7%～98.0%,测定结果的相对标准偏差为1.20%～2.09%(n=6)。该方法简单,灵敏度高且重复性好,适用于化妆品中邻伞花烃-5-醇的测定。
A method for the determination of 4-isopropyl-m-cresol in cosmetics by high performance liquid chromatography was established.Samples were extracted by methanol for 15 min,fi ltered through 0.22μm membrane,X-bridge C 18 as chromatographic column,acetonitrile-water(60∶40)as mobile phase,fl ow rate was 1.0 mL/min,column temperature was 30℃,detection wavelength was 280 nm,and detection by diode array detector.The mass concentration of 4-isopropyl-m-cresol in the range of 1.0-100.0 mg/L showed a good linear relationship with the peak area,the correlation coef fi cient was 0.999 9,the detection limit was 3.0 mg/kg and the quantitative limit was 10.0 mg/kg.The recoverie were 93.7%-98.0%,and the relative standard deviations were 1.20%-2.09%(n=6).The method is simple,sensitive and reproducible,and it is suitable for the determination of 4-isopropyl-m-cresol in cosmetics.
作者
夏泽敏
梁文耀
莫庭源
廖惠媚
熊小婷
谭建华
XIA Zemin;LIANG Wenyao;MO Tingyuan;LIAO Huimei;XIONG Xiaoting;TAN Jianhua(Guangzhou Quality Supervision and Testing Institute,National Quality Supervision and Testing Center for Cosmetics(Guangzhou),Guangzhou 511447,China)
出处
《化学分析计量》
CAS
2019年第2期76-78,共3页
Chemical Analysis And Meterage
关键词
高效液相色谱法
化妆品
邻伞花烃-5-醇
high performance liquid chromatography
cosmetics
4-isopropyl-m-cresol
