摘要
【目的】选育优良的产酸性木聚糖酶的微生物,考察酸性木聚糖酶的酶学性质(尤其是pH值为4.0),为实现纤维素乙醇低成本清洁生产打下基础。【方法】从广西大学农场采集土壤,富集后经产酸性木聚糖酶的培养,比较酸性木聚糖酶酶活力,选育酸性木聚糖酶高产菌株,鉴定菌种,分析酶学性质。【结果】筛选出产酸性木聚糖酶酶活力较高的菌株XYW5。扩增菌株XYW5的ITS rDNA序列,经测序分析比对,将其初步鉴定为日本曲霉Aspergillus japonicus XYW5。菌株XYW5产酸性木聚糖酶和酸性木糖苷酶的酶活力最高分别达(26. 26±0. 97)U/mL和(0.63±0.02) U/mL,比活力分别为(85.50±0.63) U/mg和(1.80±0.01) U/mg;其酸性木聚糖酶最适温度和最适pH值分别为65℃和6.5,酸性木糖苷酶最适温度和最适pH值分别为70℃和4.5;酸性木聚糖酶兼有酸性CMCase酶活力,达到8.54 U/mL。【结论】菌株XYW5所产的酸性木聚糖酶具有开发成为优良工业酸性木聚糖酶的潜力。
【Objective】Breeding excellent microorganisms producing acidic xylanase and investigating the enzymatic properties of acidic xylanase(especially pH 4.0)can lay the foundation for low cost and clean production of cellulosic ethanol.【Methods】The soil was collected from the farm of Guangxi University,and after enrichment,the acidic xylanase was cultured.The activity of acidic xylanase was compared,the acidic xylanase high yield strain was selected and identified,and the enzymatic properties were analyzed.【Results】XYW5 strain with high activity of acidic xylanase was screened out.The ITS rDNA sequence of XYW5 strain was amplified and initially identified as Aspergillus japonicus XYW5 by sequencing analysis and inter comparison.The enzyme activity of acidic xylanase and acidic xylosidase produced by XYW5 strain was(26.26±0.97)U/mL and(0.63±0.02)U/mL,respectively.The specific activity of the two enzymes was(85.50±0.63)U/mg and(1.80±0.01)U/mg,correspondingly.The optimum temperature and the pH value of the acidic xylanase was 65℃and 6.5,respectively.And the optimum temperature and the pH value of the acidic xylosidase was 70℃and 4.5,respectively.While acidic xylanase had acidic CMCase enzyme activity,reaching 8.54 U/mL.【Conclusion】The acidic xylanase produced by XYW5 strain has the potential to be developed into an excellent acidic xylanase for industry application.
作者
吴仁智
黄俊
芦志龙
陈小玲
陈英
陆琦
陈东
王青艳
黄日波
WU Renzhi;HUANG Jun;LU Zhilong;CHEN Xiaoling;CHEN Ying;LU Qi;CHEN Dong;WANG Qingyan;HUANG Ribo(National Engineering Research Center for Non food Bio refinery,State Key Laboratory of Non food Biomass Enzyme Technology,Guangxi Biomass Industrialization Engineering Institute,Guangxi Key Laboratory of Bio refinery,Guangxi Academy of Sciences,Nanning,Guangxi,530007,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro bioresources,College of Life Science and Technology,Guangxi University,Nanning,Guangxi,530004,China)
出处
《广西科学》
CAS
2018年第6期669-677,共9页
Guangxi Sciences
基金
国家863项目(2013AA050701)
国家科技计划课题子任务(2012AA022106)
广西重点研发计划(桂科AB16380024)
广西自然科学基金联合资助培育项目-面上项目(2018GXNSFAA138111)
广西科技开发项目(桂科合14123001-6)
广西科学院科研发展基金项目(2018YFJ402)资助
关键词
日本曲霉
筛选
酸性木聚糖酶
酶学性质
纤维素乙醇
Aspergillus japonicus
screening
acidic xylanase
enzyme properties
cellulosic ethanol
