摘要
目的:利用喷雾干燥工艺制备芽孢杆菌dhs-330-021菌粉,并研究菌粉的活性及稳定性。方法:以脱脂乳、海藻糖、β-环糊精和谷氨酸钠为保护剂,采用喷雾干燥(条件为:进口温度100℃,出口温度50～60℃,进样速度2～4mL/min)制备芽孢杆菌菌粉,以喷干存活率和菌粉活菌数为指标,选择最佳制备条件。结果:获得喷干保护剂配方为脱脂乳10.0%、海藻糖6.0%、β-环糊精13.0%、谷氨酸钠15.0%,喷干存活率为65.9%,菌粉活菌数为1.38×109CFU/g,存放180 d后菌粉活菌数为1.03×10~9CFU/g。结论:喷雾干燥工艺可以用于芽孢杆菌dhs-330-021菌粉的制备,获得的菌粉稳定性较好。
Objective:Fermentation broth of Bacillus sp.dhs-330-021 was prepared by spray drying technology.The activity and stability of bacterial power were researched.Methods:The bacterial power of Bacillus was prepared by spray drying technology at the inlet temperature of 100℃,outlet temperature 50~60℃,and injective flow rate 2~4 mL/min.The protective agents were skim milk,trehalose,β-cyclodextrin and sodium glutamate.The optimal conditions were determined by survival rate and viable counts of bacterial power.Results:The optimal formula of protective agents for spray drying were skim milk 10.0%,trehalose 6.0%,β-cyclodextrin 13.0% and sodium glutamate 15.0%.Under the optimal conditions,the survival rate was 65.9%,the viable counts were 1.38×10^9 CFU/g,and the viable counts were 1.03×10^9 CFU/g after storage for 180 days.Conclusion:Spray drying technology can be used for the preparation of Bacillus sp.dhs-330-021 powder with better stability.
作者
司晓光
张晓青
杜瑾
张爱君
曹军瑞
SI Xiao-Guang;ZHANG Xiao-Qing;DU Jin;ZHANG Ai-Jun;CAO Jun-Rui(Institute of Seawater Desalination and Multipurpose Utilization,Ministry of Natural Resources,Tianjin 300192,China)
出处
《生物技术通讯》
CAS
2019年第1期85-88,共4页
Letters in Biotechnology
基金
中央级公益性科研院所基本科研业务费专项(K-JBYWF-2017-G13
K-JBYWF-2017-G14)
海洋公益性行业科研专项(201505032-2)
关键词
芽孢杆菌
菌粉
保护剂
喷雾干燥
Bacillus sp
bacterial powder
protective agents
spray drying
