摘要
为研究不同因素对滤食性贝类碳氮稳定同位素分馏的影响,选取物种(虾夷扇贝Patinopecten yessoensis、海湾扇贝Argopecten irradians、太平洋牡蛎Crassostrea gigas)、温度(6、14、23℃)和饵料(新月菱形藻Nitzschia closterium、小球藻Chlorella vulgaris、叉鞭金藻Dicrateria inornata)3因素进行正交试验,通过检测3种贝类及饵料的稳定同位素变化,探讨了3种因素对滤食性贝类碳氮稳定同位素分馏的影响。结果表明:饵料的氮稳定同位素比值(δ^(15)N)波动较大,无法满足试验要求;各因素对碳稳定同位素半衰期(t_(1/2))的影响由大到小均为温度>物种>饵料,但无显著性差异(P>0.05),对碳稳定同位素判别值(Δ^(13)C)的影响程度极为接近,Δ^(13)C的变化范围为-5.57‰～1.75‰,已超出经验值范围。研究表明:在以微藻为饵料的稳定同位素判别值研究中,要谨慎选择氮稳定同位素作为参考指标;应用稳定同位素法研究食物网的营养关系时,需全面、系统考虑综合因素对判别值的影响。
In an orthogonal test, yesso scallop Patinopecten yessoensis with shell height of (0.69±0.11)cm, bay scallop Argopecten irradians with shell height of (0.82±0.08)cm, and Pacific oyster Crassostrea gigas with shell height of (1.02±0.19)cm were monocultured in a 10 L glass fiber reinforced plastic tank and fed algae including Nitzschia closterium, Chlorella vulgaris and Dicrateria inornata at water temperatures of 6, 14 ℃ and 23 ℃ for 62 days. Then the changes in values of stable carbon and nitrogen isotopes were monitored 0, 11, 22, 26, 38, 50 days after rearing in the filter-feeding mollusks and food algae to investigate the effects of these three factors (temperature, diet and mollusk species)on the stable carbon and nitrogen isotopes discrimination. The variation of the δ^15 N values in diets was so large that did not meet the need for the experiment requirement. The water temperature was the most important factor influencing the t 1/2 of carbon, followed by mollusk species and diet, without significant differences in these three factors ( P >0.05), and quite similar effect of the three factors on the Δ^13 C. The value of Δ^13 C was changed from -5.57‰ to -1.75‰ in the unaccepted range in the experiment. Therefore, it needs to be careful to select the nitrogen stable isotope as reference indicator when the microalgae are used as diet to investigate the stable isotope discriminative values. The effects of comprehensive factors on the discriminant values should be comprehensively and systematically considered using the stable isotope to understand the food web.
作者
田甲申
王震
李多慧
鹿志创
TANG Xiao-feng;LIU Li-ping;SHUAI Dian;ZHANG Li-na(Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2019年第1期80-86,共7页
Journal of Dalian Ocean University
基金
辽宁省海洋与渔业厅科研项目(201609)
关键词
滤食性贝类
碳氮稳定同位素
分馏
影响因素
filter-feeding mollusk
stable carbon and nitrogen isotope
fractionation
influence factor
