摘要
目的建立广州地区精液常规分析的生物参考区间,为临床不育症诊治和患者生育力评估提供科学的实验室判断依据。方法收集2015年2月至2016年5月正常体检男性(2年内已生育、准备要二孩)精液标本156份,使用西班牙SCA全自动精子分析仪及改良巴氏染色法,根据第5版《世界卫生组织人类精液检查与处理实验室手册》,检测精液主要指标及正常精子形态率,按美国临床和实验室标准协会(NCCLS)的标准化文件C28-A2进行统计处理,建立起该实验室精液分析的生物参考区间。结果该实验室精液分析的生物参考区间结果如下:精液量≥2.00mL;液化时间≤45.00min;pH值为7.0～8.0;精子浓度为≥17.95×10~6/mL;前向运动精子总数为≥24.15×10~6/一次射精;精子总活动率为≥36.71%;精子前向活动率≥28.10%;精子正常形态率≥3.00%。结论实验室应建立所在区域人群精液分析的生物参考区间,为临床不育症诊治或辅助生殖技术实施提供科学的实验室判断依据。
Objective To establish the biological reference intervals of semen analysis in Guangzhou area to provide a scientific laboratory judgment basis for the clinical diagnosis and treatment of clinical infertility and fertility evaluation.Methods A total of 156 cases of semen sample from normal males undergoing physical examination(having birth within two years and preparing the second baby)from February 2015 to May 2016 were collected.The Spanish SCA automatic sperm analyzer and improved pap staining were used to detect the main indicators of semen and rate of normal sperm morphology according to the fifth edition of the WHO Laboratory Manual for Human Semen Examination and Treatment.The statistical process was performed according to the standardized documents C28-A2 of NCCLS.Then the biological reference intervals of semen analysis in this laboratory were established.Results The biological reference intervals of semen analysis in this laboratory are as follows:the semen volume≥2.00 mL,the liquefaction time 45.00 min,the pH value 7.0-8.0,the sperm concentration 17.95×10^6/mL,the total number of moving sperm 24.15×10^6/one time,the sperm total motility≥36.71%,the sperm progressive motility 28.10%,the rate of sperm normal morphology 3.00%.Conclusion The laboratory establishes the normal biological reference intervals of semen analysis in local area to provide a scientific laboratory judgment basis for the clinical diagnosis and treatment of clinical infertility or assisted reproductive technology implementation.
作者
石汉振
杨佩桦
曾冬玉
SHI Hanzhen;YANG Peihua;ZENG Dongyu(Department of Clinical Laboratory,Guangdong Provincial Hospital of Traditional ChineseMedicine,Guangzhou,Guangdong 510530,China;Jingyu Clinical Laboratory Institute,Guangzhou Medical University,Guangzhou,Guangdong 511436,China;Department of Infection,First Affiliated Hospital of Medical College,Jinan University,Guangzhou,Guangdong 510120,China)
出处
《检验医学与临床》
CAS
2019年第5期581-584,共4页
Laboratory Medicine and Clinic
基金
广东省中医院朝阳人才计划项目(E42703)
关键词
生物参考区间
精液
精液参数
biological reference intervals
semen
semen parameters
