摘要
目的探讨解脲脲原体(Uu)感染对男性不育患者精子膜蛋白DCXR表达和精子凋亡的影响。方法参照世界卫生组织(WHO)标准方法对145例精液标本进行精液常规分析。Western blot和免疫荧光分别检测人精子DCXR蛋白的表达和定位,采用TUNEL法检测精子凋亡。对Uu感染阳性者进行敏感抗生素治疗,分析治疗前、后的不育症患者精液各项指标的变化。结果 Uu阳性不育组精子DCXR/β-actin平均光密度和精子DCXR阳性率均低于与Uu阴性不育组(P <0.05),治疗后Uu阳性不育组的DCXR/β-actin平均光密度和精子DCXR阳性率均明显高于治疗前Uu阳性不育组,差异有统计学意义(P <0.05)。Uu阳性不育组的精子凋亡率高于与Uu阴性不育组,治疗后Uu阳性不育组的精子凋亡率明显低于治疗前Uu阳性不育组,差异有统计学意义(P <0.05)。相关性分析提示精子DCXR蛋白表达量和精子DCXR阳性率分别与精子凋亡率呈负相关。结论 Uu感染阳性导致精子DCXR蛋白表达减少、精子DCXR阳性率降低和精子凋亡率升高,Uu感染合理治疗后精子DCXR蛋白表达和精子凋亡有明显改善;精子DCXR蛋白的表达具有保护精子并减少精子凋亡率的作用。
Objective To study the effect of Ureaplasma urealyticum on the level of DCXR expression and apoptosis in human spermatozoa. Methods 145 cases were in infertile group. Semen routine analysis was referred to the WHO standard method. Western blot was used to detect the level of DCXR expression in spermatozoa. The localization of the protein in human spermatozoa was determined by indirect immunofluorescent staining. The sperm apoptosis was assayed by TUNEL. Analysis the indicators of change before and after treatment with sensitive antibiotics for Uu infection. Results The expression of DCXR protein and the percentage of DCXR-positive rate were lowerin UU positive group than those in UU negative group. The expression of DCXR protein and the percentage of DCXR-positive rate were higher after treatment,than before therapy in Uu positive group(P < 0.05). The sperm apoptosis rate in Uu positive group were higher than those in Uu negative. The sperm apoptosis rate was lower after treatment than before therapy in Uu positive group(P < 0.05). Conclusion Ureaplasma urealyticum infection may affect the level of DCXR protein expression,the percentage of the DCXR-positive rate and sperm apoptosis in human spermatozoa . After effective treatment of Uu infection,the sperm protein DCXR expression and sperm apoptosis would be obviously improved.
作者
马晓萍
岳应权
高晓勤
MA Xiaoping;YUE Yinquan;GAO Xiaoqin(Department of Histology and Embryology,Zunyi Medical andPharmaceutical College,Zunyi 563006,China)
出处
《实用医学杂志》
CAS
北大核心
2019年第3期407-411,共5页
The Journal of Practical Medicine
基金
贵州省科技计划项目(编号:黔科合LH字[2015]7568号)
遵义市科技项目(编号:遵市科合社字[2018]35号)
