摘要
目的:通过检测不育症患者精浆中高迁移率族蛋白B1(HMGB1)、C-反应蛋白的含量,探讨HMGB1、C-反应蛋白在不育症患者中的临床意义。方法:采用酶联免疫吸附试验对61例不育(其中梗阻型无精子症18例)患者、20例正常生育男性精浆中HMGB1进行检测,C-反应蛋白采用散射比浊法检测。结果:无精子症患者精浆中HMGB1[(22.68±4.45)μg/L]、C-反应蛋白[(33.71±6.96)mg/L]含量显著高于正常生育组[(12.88±6.84)μg/L;(1.73±0.78)mg/L]及一般不育组[(14.68±8.25)μg/L;(2.34±1.78)mg/L];无精子症患者精浆HMGB1水平与C-反应蛋白水平有很好的相关性,相关系数为0.97。结论:无精子症患者精浆炎性介质HMGBl水平明显升高,可能与炎症免疫反应有关。
Objective To investigate the clinical significance of high mobility group box 1 （HMGB1） and C-reactive protein in seminal plasma in male sterility. Methods The levels of HMGB1 in seminal plasma of 61 infertile men （including 18 aspermias）and normal 20 males were detected by ELISA. The levels of C-reactive protein were detected with nephelometry immunoassay. Results The level of HMGB1（22.68 ± 4.45）μg/L and C-reactive protein（33.71 ± 6.96）mg/L in seminal plasma of 18 aspermias were significantly higher than that in normal males[（12.88 ± 6.84）μg/L;（1.73 ± 0.78）mg/L] and other infertile men [（14.68 ± 8.25）μg/L;（2.34 ± 1.78）mg/L]. The correlation coefficient was 0.97 between HMGB1 and C-reactive protein levels of seminal plasma in aspermias. Conclusion The level of HMGB1 is significantly elevated in seminal plasma of aspermias, which may be related with its inflammatory response.
出处
《实用医学杂志》
CAS
北大核心
2012年第5期726-728,共3页
The Journal of Practical Medicine
基金
国家自然科学基金资助项目(编号:81070074)
