摘要
目的:通过检测慢性乙型肝炎患者外周血单个核细胞(PBMCs)高迁移率族蛋白1(HMGB1) mRNA的表达情况,研究HMGB1在乙型肝炎中的临床意义．方法:应用逆转录聚合酶链反应(RT-PCR) 对54例慢性乙型肝炎患者和10例健康对照者PBMCs HMGB1 mRNA的表达进行检测, 同时应用ELISA法检测外周血浆肿瘤坏死因子α(TNF-α)和内毒素(LPS)水平,比较各组HMGB1 mRNA表达水平的差异及其与 TNF-α、LPS、总胆红素(TBIL)、凝血酶原活动度(PTA)的关系．结果:PBMCs HMGB1 mRNA的表达水平在慢性重型肝炎组分别高于慢性肝炎组(0．89± 0．06 vs 0．70±0．10,P<0．01)和正常对照组 (0．89±0．06 vs 0．58±0．08,P<0．01),慢性肝炎组高于正常对照组(0．70±0．10 vs 0．58±0．08． P<0．01)．在23例慢性重型肝炎患者中,11例未恢复患者PBMCs HMGB1 mRNA表达水平明显高于12例恢复的患者(0．93±0．04 vs 0．85± 0．05,P<0．01)．其中12例已恢复的慢性重型肝炎患者,患者恢复期PBMCs HMGB1 mRNA 的表达水平较发病期明显下降(0．72±0．07 vs 0．85±0．05,P<0．01)．HMGB1 mRNA表达水平与外周血浆LPS、TNF-α及TBIL均呈显著正相关(r=0．74,0．64,0．71,均P<0．01),与PTA呈负相关(r=-0．82．P<0．01)．结论:HMGB1在乙型肝炎发病过程中可能起重要作用,HMGB1 mRNA的表达水平高低与病情轻重密切相关．
AIM： To investigate the expression and clinical significance of high mobility group box-1 protein （HMGB1） mRNA in chronic hepatitis B. METHODS： The expression of HMGB1 mRNA was detected in peripheral blood mononuclear cells （PBMCs） of 54 patients with chronic hepatitis B and 10 normal controls by reverse transcription-polymerase chain reaction （RT- PCR）. The levels of plasma tumor necrosis factor-α （TNF-α） and lipopolysaccharide （LPS） were measured by enzyme linked immunosorbent assay （ELISA） simultaneously. The levels of total bilirubin （TBIL） prothrombin time activity percentage （PTA） were examined by biochemical methods. RESULTS： HMGB1 mRNA expression was significantly increased in severe hepatitis patients in comparison with that in chronic hepatitis B patients and normal controls （0.89±0.06 vs 0.70±0.10, 0.58±0.08, P 〈0.01）. Marked difference also existed between the latter two （0.70±0.10 vs 0.58±0.08, P 〈0.01）. Of the 23 patients with chronic severe hepatitis, the HMGB1 mRNA levels in 11 unrecovered patients were notably higher than those in 12 recovered patients （0.93±0.04 vs 0.85±0.05, P 〈0.01）. Of the 12 recovered patients, the levels of HMGB1 mRNA were significantly decreased in the stage of recovery as compared with those in the stage of onset （0.72±0.07 vs 0.85±0.05, P 〈0.01）. There was positive correlation among HMGB1 mRNA expression and plasma LPS, TNF-α and TBIL （r = 0.74, 0.64, 0.71 respectively, P〈0.01） and negative correlation between HMGB1 mRNA expression and PTA （r = -0.82, P 〈0.01）. CONCLUSION： HMGB1 plays an important role in the pathogenesis of chronic hepatitis B, and HMGB1 mRNA level is closely associated with the condition the disease.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第2期167-172,共6页
World Chinese Journal of Digestology
