摘要
蛋白水解物是蛋白质经酶、酸、碱和发酵等方法水解得到的混合物,由于其含有丰富的氨基酸和多肽,并为细胞生长提供多种营养成分、贴壁因子及生长因子类似物等,从而在生物技术领域中得到了广泛的应用。本文综述了蛋白水解物制备工艺、在生物技术领域中的应用及其在细胞培养中对细胞增殖、代谢的影响,以期为蛋白水解物的开发和应用提供参考。
Protein hydrolysates,a mixture of amino acids and peptides,were prepared through the hydrolysis of proteins by enzyme,acid,alkali and fermentation methods.Protein hydrolysates had been widely used in the field of biotechnology due to that the hydrolyzatescould provide a variety of nutrients,adherent factors and growth factor analogs for cell growth.This review summarized the preparation of protein hydrolysates,the application of hydrolysates in the field of biotechnology,and the effects of hydrolysates on cell culture,proliferation and metabolism in order to provide basis for the further study and application of protein hydrolysates.
作者
张邵博
靳冬武
李明生
ZHANG Shao-bo;JING Dong-wu;LI Ming-sheng(Life Science and Engineering College of Northwest University for Nationalities;Engineering Technology Research Center for Animal Cell;Lanzhou MinHai Bio-Engineering Co.,Ltd,Lanzhou 730030,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第2期354-362,共9页
Natural Product Research and Development
基金
甘肃省科技计划(17YF1WA166)
甘肃省科技计划(1504WKCA094)
科技部科技援助项目(KY201501005)
中国-马来西亚清真食品国家联合实验室开放基金(CM2017001)
关键词
蛋白水解物
制备工艺
生物领域
细胞培养
应用
protein hydrolysates
preparation process
application in biotechnology fields
cell culture
