摘要
目的观察一种新型高脂血症易感WSHc大鼠经高脂饲料诱导后血脂的动态变化和心血管并发症病理特点,为WSHc大鼠的应用提供参考。方法取7～8周龄雌性WSHc大鼠20只和Wistar大鼠10只,进行高脂饲喂,另取同周龄同性别的WSHc大鼠和Wistar大鼠各10只饲喂普通饲料,分别作为正常对照。观察高脂饲喂2、4、8、12、16周时的血脂动态变化,饲喂16周后采用心动超声检测大鼠心功能及左心结构,病理组织学观察心脏及主动脉病变。结果与正常对照组比,高脂饲喂2周后WSHc大鼠与Wistar大鼠血清总胆固醇(TC)与低密度脂蛋白胆固醇(LDL-c)均显著升高;高脂饲喂的WSHc大鼠TC、LDL-c水平为:(6.34±2.12)mmol/L、(2.56±0.94)mmol/L,而高脂饲喂的Wistar大鼠TC、LDL-c水平仅为(2.93±0.23)mmol/L,(0.63±0.12)mmol/L;在高脂饲喂期间,WSHc大鼠TC始终维持在高水平状态。饲喂16周后,WSHc大鼠心脏射血分数升高,左心室室壁增厚,并出现心肌纤维化,心肌细胞凋亡程度增高;主动脉内膜增厚,弹力纤维排列紊乱,形成早期动脉粥样硬化病变。而Wistar大鼠经高脂饲料诱导后,心功能及心血管病变不明显。结论 WSHc大鼠对外源性胆固醇敏感,高脂饲喂后易发高脂血症及心血管疾病,且TC、LDL-c水平与临床高脂血症更接近,相较于普通大鼠,更适用于高脂血症及相关心血管并发症的实验研究。
Objective This study aimed to observe the dynamic variation of blood lipids and the pathological features of cardiovascular complications induced by a high-fat diet in a new type of hyperlipidemia-susceptible (WSHc) rats, and to provide a reference for the application of WSHc rats. MethodsTwenty female WSHc rats and 10 Wistar rats, aged 7-8 weeks, were fed with a high-fat diet. Another 10 WSHc and Wistar rats of the same age and sex were given a normal diet as a control group. Dynamic changes of blood lipids in the rats were observed at 2, 4, 8,12, and 16 weeks. After feeding for 16 weeks, the cardiac function of the rats in each group was tested using a cardiac ultrasound, and pathological changes of the heart and aorta were observed by histopathology. ResultsAfter 2 weeks of the high-fat diet feeding, the serum total cholesterol (TC) of WSHc and Wistar rats was significantly higher than that of the control group. The levels of TC and LDL-c in high-fat-diet-fed WSHc rats were (6.34±2.12) and (2.56±0.94) mmol/L, while the levels in the Wistar rats fed with high fat diet were only (2.93±0.23) and (0.63±0.12) mmol/L, respectively;during high-fat diet feeding, the TC of WSHc rats remained at a high level. After 16 weeks of high-fat diet intervention, the cardiac ejection fraction of the WSHc rats was increased. Furthermore, the left ventricular wall was thickened, and myocardial fibrosis occurred. Additionally, the aortic intima was thickened, and the arrangement of elastic fibers was disordered, forming early atherosclerotic lesions. However, lesions and cardiovascular disease were not apparent in the Wistar rats after the high-fat diet. ConclusionsWSHc rats are sensitive to exogenous cholesterol, and are prone to hyperlipidemia and related cardiovascular complications after high-fat diet feeding. Additionally, the TC and LDL-c levels are close to those in clinical hyperlipidemia. Compared with normal rats, they are more suitable for studying hyperlipidemia and related cardiovascular complications.
作者
马全鑫
陈姣姣
郁晨
张利棕
寿旗扬
陈民利
MA Quanxin;CHEN Jiaojiao;YU Chen;ZHANG Lizong;SHOU Qiyang;CHEN Minli(Animal Experimental Research Center/Institute of Comparative Medicine, Zhejiang Chinese Medical University,Hangzhou 310053, China)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2019年第1期25-31,共7页
Acta Laboratorium Animalis Scientia Sinica
基金
国家卫生计生委科学研究基金-浙江省医药卫生重大科技计划项目(2016149056)
浙江省科技厅公益性(实验动物平台)项目(2016C37107)~~
