摘要
脊髓灰质炎野毒株消灭后,口服脊髓灰质炎减毒活疫苗(Oral polio vaccine,OPV)将被停止使用,脊髓灰质炎灭活疫苗(Inactivated poliovirus vaccine,IPV)将全面替代OPV,但IPV成本过高,难以满足全球需要。皮内免疫可以降低Sabin株脊髓灰质炎灭活疫苗(Inactivated poliovirus vaccine derived from Sabin strain,sIPV)的免疫剂量,本研究将观察sIPV疫苗皮内免疫大鼠后的免疫持久性及加强免疫效果。本研究采用sIPV,设皮内免疫组、全剂量肌肉免疫组和皮内免疫阴性对照组,接种Wistar大鼠,于3剂基础免疫程序完成后第1个月、12个月采血;第12个月采血后加强免疫1剂,并于加强免疫1个月后采血。中和试验检测各血清抗脊灰病毒中和抗体效价,评价皮内免疫sIPV的免疫持久性及加强免疫效果。Wistar大鼠3剂基础免疫后1个月,1/5、1/3剂量皮内免疫组与全剂量肌肉免疫组Ⅰ、Ⅱ、Ⅲ型抗体阳转率均达到了100%,各型别中和抗体几何平均滴度(Geometric mean titer,GMT)均远高于1∶8保护水平。基础免疫后12个月,sIPV全剂量组各型阳转率均维持在80%以上,1/10剂量皮内免疫组在50%以上,1/5剂量皮内免疫组维持在70%以上,1/3剂量皮内免疫组维持在80%以上,除1/10剂量组Ⅱ型外其余各组各型别GMT均维持在1∶8以上。加强免疫后1个月,1/5剂量皮内免疫组、1/3剂量皮内免疫组及全剂量组的Ⅰ型、Ⅱ型、Ⅲ型各组中和抗体阳转率均达到100%,并能够诱导产生远高于1∶8的抗体水平。本研究结果显示sIPV疫苗皮内免疫具有良好的免疫持久性及加强免疫效果。
After the elimination of wild poliovirus strains, oral polio vaccine( OPV) will be no longer used. Inactivated poliovirus vaccine(IPV) will replace OPV worldwide. However, the cost of IPV is too high to meet the global needs. Intradermal immunization can reduce the dosage of inactivated poliovirus vaccine derived from the Sabin strain(sIPV). To observe the immune persistence and efficacy of boosting dose of sIPV after intradermal immunization in rats, full-dose sIPV given via the intramuscular route was used as control. Experimental and negative-control groups were designed as which were injected with sIPV and saline, respectively, via the intradermal route. Wistar rats in various groups were immunized and blood samples were collected at the first and 12th month after completion of three doses of the basic immunization program. After blood collection at the 12th month post-immunization, one boosting dose was immunized. Blood samples were collected after 1 month of using the boosting dose. Titers of neutralizing antibodies were tested to evaluate the immune persistence and efficacy of a boosting dose of sIPV. One month after a three-dose schedule in Wistar rats, all of the seroconversion rates of types Ⅰ, Ⅱ and Ⅲ of 1/5-dose, 1/3-dose needle-free intradermal group, and full-dose intramuscular group were 100%. The geometric median titers(GMTs) of all three types were much higher than that of the protective titer(1 : 8). Twelve months after the basic immunization, the seroconversion rates of all types were great than 80% in the full-dose sIPV group, great than or equal to 50% in the 1/10-dose intradermal group,great than or equal to 70% in the 1/5-dose intradermal group,and great than or equal to 80% in the 1/3-dose intradermal group. GMTs of all three types were maintained great than 1: 8 in all groups except type Ⅱ in the 1/10-dose intradermal group. One month after the boosting dose, all of the seroconversion rates of types Ⅰ, Ⅱ and Ⅲreached 100% in the 1/5-dose intradermal group, 1/3-dose intraderm
作者
马磊
寸怡娜
苏敏
宋绍辉
蔡玮
周健
欧阳圣洁
刘婧
胡文著
李卫东
廖国阳
MA Lei;CUN Yina;SU Min;SONG Shaohui;CAI Wei;ZHOU Jian;OUYANG Shengjie;LIU Jing;HU Wenzhu;LI Weidong;LIAO Guoyang(Institute of Medical Biology,Chinese Academy of Medical Sciences,Peking Union Medical College,Kunming 650118,China;The First People's Hospital of Yunnan Province,Kunming 650032,China)
出处
《病毒学报》
CAS
CSCD
北大核心
2019年第1期6-11,共6页
Chinese Journal of Virology
基金
国家国际科技合作专项项目(项目号:2014DFA32870)
题目:Sabin-IPV无针注射皮内免疫减少剂量合作研究
协和创新团队项目(项目号:2016-I2M-3-026)
题目:疫苗关键技术创新团队
云南省国际科技合作项目(项目号:2017IB008)
题目:替代活病毒中和试验评价脊髓灰质炎疫苗免疫效果和监测人体免疫保护力的关键技术国际合作研究
云南省创新团队(项目号:2015HC027)
题目:中国医学科学院医学生物学研究所疫苗研发与新技术应用研究省创新团队
云南省应用基础研究项目(项目号:2015FB097)
题目:云南迪庆高原地区藏族和汉族健康人群血细胞分析参考区间的建立和研究~~
