摘要
目的基于"肾主骨"理论分析左归丸对大鼠破骨细胞(OC)凋亡的影响。方法选取15只雌性健康Wistar大鼠,按照随机数字表法随机将其分为卵巢切除(OVX)加左归丸组、OVX组及对照组各5只,给予相关处理后,制备相应血清。取1日龄Wistar乳鼠制备大鼠OC及成骨细胞(OB)体外分离和培养,并分别分为6组和3组与上述血清共同培养,检测比较前6组OC所引起骨吸收陷窝数目和面积,以及后3组OB骨保护素(OPG)及破骨细胞核因子k B受体活化因子配体(RANKL)蛋白表达。结果随着培养时间延长,各组骨吸收陷窝数目及面积随之增大。培养第7天时,各组骨吸收陷窝数目及面积总体比较差异均有统计学意义(P <0. 05)。培养第7天时,OC+OVX血清组骨吸收陷窝数目及面积均高于OC+OVX含药血清组及OC+正常血清组,OC+OVX含药血清组骨吸收陷窝数目及面积均高于OC+正常血清组; OC+OB+OVX血清组骨吸收陷窝数目及面积均高于OC+OB+OVX含药血清组及OC+OB+正常血清组; OC+OB+OVX血清组骨吸收陷窝数目及面积均高于OC+OVX血清组,OC+OB+OVX含药血清组骨吸收陷窝数目及面积均低于OC+OVX含药血清组,差异有统计学意义(P <0. 05)。各组OB OPG及RANKL蛋白表达总体比较差异有统计学意义(P <0. 05)。OB+OVX血清组OB OPG蛋白表达明显低于OB+正常血清组,OB RANKL蛋白表达明显高于OB+正常血清组; OB+OVX含药血清组OB OPG蛋白表达高于OB+OVX血清组,OB RANKL蛋白表达低于OB+OVX血清组,差异有统计学意义(P <0. 05)。结论左归丸含药血清可直接抑制OC,还可通过OB间接抑制OC,作用机制可能与调节OB OPG、RANKL蛋白表达相关,提示基于"肾主骨"理论的左归丸可治疗骨质疏松症。
Objective To investigate the effect of Zuogui Pill on apoptosis of osteoclasts(OC)in rats based on the Theory of Kidney Dominating the Bone.Methods Fifteen healthy female Wistar rats were selected and randomly divided into three groups:ovariectomy(OVX)+Zuogui Pill group(n=5),OVX group(n=5)and the control group(n=5)according to random number table method.After relevant treatment,the corresponding serum was prepared.OC and osteoblasts(OB)of rats were isolated from tibia,tibia,femur and skull of 1 day-old Wistar rats and cultured.They were divided into 6 groups and another 3 groups and co-cultured with the above serum.The number and area of bone resorption cavities caused by OC were compared in the first six groups.In addition,expression of OB osteoprotegerin(OPG)and receptor activator of nuclear factor kappa-Β ligand(RANKL)of OC was detected and compared in another 3 groups.Results With the extension of culture time,the number and area of bone resorption cavities were increased.At the 7 th day after culture,there were significant differences in the number and area of bone resorption cavities(P<0.05).At the 7 th day after culture,the number and area of bone resorption cavities in OC+OVX serum group were larger than those in OC+OVX drug-containing serum group and OC+normal serum group(P<0.05),and those in OC+OVX drug-containing serum group were larger than those in OC+normal serum group.The number and area of bone resorption cavities in OC+OB+OVX serum group were larger than those in OC+OB+normal serum group and OC+OB+OVX drug-containing serum group,those indicators were larger in OC+OB+OVX serum group than in OC+OVX serum group,while those were smaller in OC+OB+OVX drug-containing serum group than in OC+OVX drug-containing serum group(P<0.05),there were significant differences in expressions of OB OPG and RANKL protein(P<0.05).The expression of OB OPG protein was lower in OB+OVX serum group than in OB+normal serum group,while expression of OB RANKL protein was significantly higher than OB+normal serum group(P<0.05)
作者
李强
刘霞
陈勇
闫威
范龙坤
马超
刘国林
郭燕军
王沐
LI Qiang;LIU Xia;CHEN Yong;YAN Wei;FAN Long-kun;MA Chao;LIU Guo-lin;GUO Yan-jun;WANG Mu(Department of Oral and Maxillofacial Surgery, the Central Hospital of Cangzhou, Cangzhou, Hebei 061001, China;Department of Basic Medicine, Cangzhou Medical College, Cangzhou, Hebei 061001, China;Department of Pharmacy, the Fourth People's Hospital of Langfang, Langfang, Hebei 065000, China)
出处
《临床误诊误治》
2019年第2期94-98,共5页
Clinical Misdiagnosis & Mistherapy
基金
河北省科学技术厅科技支撑项目(20151636)
沧州市科技局科技支撑计划项目(151302160)
关键词
“肾主骨”理论
左归丸
大鼠
破骨细胞
The Kidney Being in Charge of Bone Theory
Left to pill
Rats
Osteoclasts
