摘要
目的研究脑衰反应调节蛋白2(CRMP-2)在单眼形觉剥夺性弱视大鼠视皮层的表达。方法出生后14d的SD健康大鼠64只,采用随机数字表法分为单眼形觉剥夺性弱视组和正常对照组。单眼形觉剥夺性弱视组于出生后14d行右眼睑缘缝合术,单眼形觉剥夺性弱视组和正常对照组分别于出生后14、21、45和120d各取8只大鼠进行观察。在出生后21d(单眼剥夺7d)对大鼠行闪光视觉诱发电位(F-VEP)检测,记录P1波的潜伏期和波幅值。利用免疫组织化学检测法观察2个组大鼠视皮层中CRMP-2免疫阳性神经元的表达变化,通过图像分析系统测定平均吸光度(A)值。结果F-VEP检查结果显示,与正常对照组相比,形觉剥夺性弱视组P1波潜伏期延长,波幅降低,差异均有统计学意义(t=16.760,P=0.000;t=-22.919,P=0.000)。免疫组织化学检测显示,单眼形觉剥夺性弱视组和正常对照组大鼠出生后不同时间点视皮层中CRMP-2的表达量比较,差异均有统计学意义(F分组=8.855,P=0.010;F时间=63.091,P=0.000),其中出生后21、45和120d单眼形觉剥夺性弱视组CRMP-2的表达量较正常对照组明显降低,差异均有统计学意义(均P<0.05)。结论CRMP-2可能参与单眼形觉剥夺性弱视的发生和发展过程,但其具体作用机制还有待进一步研究。
Objective To study the expression of collapsin response mediator protein 2(CRMP-2)in the visual cortex of monocular form deprivation amblyopia rats.Methods Sixty-four 14-day-old rats were randomly divided into monocular deprivation amblyopia group and normal control group by random number table method.Right eyelid margin suture was performed at 14 days after birth in the monocular deprivation amblyopia group.Eight rats in the monocular deprivation amblyopia group and the normal control group were observed at 14,21,45 and 120 days after birth,respectively.Flash visual evoked potential(F-VEP)was used to dectect the latency and amplitude of P 1 wave.The expression of CRMP-2 in visual cortex was observed by immunohistochemical method.The use and care of the animals complied with Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.This study protocol was approved by Ethic Committee of the University of South China(No.20140228).Results F-VEP results showed that the amplitudes of P 1 were decreased and latent periods of P 1 were prolonged in the monocular deprivation amblyopia group compared with the normal control group(t=16.760,P=0.000;t=-22.919,P=0.000).CRMP-2 expression levels in the visual cortex of monocular deprivation amblyopia groups and normal control groups were compared at different time points after birth,and the differences were statistically significant(F group=8.855,P=0.010;F time=63.091,P=0.000).Compared with normal control groups,the expressions of CRMP-2 at the postnatal 21,45 and 120 days were obviously decreased in the monocular deprivation amblyopia groups,the differences were statistically significant(all at P<0.05).Conclusions CRMP-2 may be involved in the occurrence and development of amblyopia.
作者
王智
袁学双
费志刚
肖启国
夏世刚
Wang Zhi;Yuan Xueshuang;Fei Zhigang;Xiao Qiguo;Xia Shigang(Department of Ophthalmology,the Second Affiliated Hospital of University of South China,Hengyang 421001,China)
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2019年第2期88-92,共5页
Chinese Journal Of Experimental Ophthalmology
基金
湖南省卫生健康委员会医药卫生科研计划项目(B2012-053)
衡阳市科技局社会发展科技支撑计划项目(2012KS4).
关键词
脑衰反应调节蛋白2
形觉剥夺性弱视
大鼠
视皮层
Collapsin response mediator protein 2
Form deprivation amblyopia
Rat
Visual cortex