摘要
目的研究血清Pentraxin-3(PTX-3)与肿瘤相关糖类抗原(CA)724和CA199在胃癌、胃良性病变患者及健康人群中的水平,探讨其表达在胃癌诊断中的临床应用价值。方法采取电化学发光法检测80例胃癌患者(胃癌组)、80例胃良性病变患者(胃良性病变组)及40例健康者(健康对照组)血清中CA724和CA199水平,采用酶联免疫吸附试验(ELISA)检测所有研究对象血清中PTX-3水平,并分析各指标水平与胃癌患者临床病理参数之间的相关性,同时比较这3项指标联合检测对胃癌的诊断价值。结果胃癌组患者血清中CA724、CA199和PTX-3水平明显高于胃良性病变组(P<0.05)和健康对照组(P<0.01);血清中CA724、CA199和PTX-3水平均与胃癌的TNM分期、分化程度、淋巴结转移与否密切相关(P<0.05)。血清CA724、CA199和PTX-3单独检测时,灵敏度均较低,特异度较高。而多项血清指标联合检测时,CA724+CA199+PTX-3联合检测对于胃癌的诊断效果最佳,其灵敏度为95.00%,特异度为79.17%,准确度为93.00%,约登指数为0.77。结论胃癌患者血清CA724、CA199和PTX-3水平显著增加,可能参与了胃癌的发生、发展,这3项指标的联合检测可以提高临床上胃癌诊断的准确性,具有重要的临床应用价值。
Objective To study the levels of serum Pentraxin-3(PTX-3)and tumor-associated carbohydrate antigens CA724 and CA199 in the patients with gastric cancer,benign gastric lesions and normal people,and to explore the clinical application value of their expressions in the diagnosis of gastric cancer.Methods The levels of tumor-associated carbohydrate antigens CA724 and CA199 in 80 patients with gastric cancer,80 patients with benign gastric lesions and 40 normal controls were detected by adopting the electrochemiluminescence.The level of serum PTX-3 in all patients was detected by adopting the enzyme-linked immunosorbent assay(ELISA).Then the correlation between the levels of various indexes and clinicopathological parameters in the patients with gastric cancer was analyzed.Meanwhile the values of combined detection among 3 indexes for diagnosing gastric cancer were compared.Results The levels of serum CA724,CA199 and PTX-3 in the gastric cancer group were significantly higher than those in the benign gastric lesions(P<0.05)and healthy control group(P<0.01).The levels of serum CA724,CA199 and PTX-3 had the close correlation with the TNM stage,differentiation degree and lymph node metastasis(P<0.05).In the single detection of serum CA724,CA199 and PTX-3,the sensitivity was lower,while the specificity was higher.In the combined detection of multiple serum indicators,the combined detection of CA724+CA199+PTX-3 was the best for the diagnosis of gastric cancer.The sensitivity was 95.00%,the specificity was 79.17%,the accuracy was 93.00%,and YI was 0.77.Conclusion The levels of serum CA724,CA199 and PTX-3 in the patients with gastric cancer are significantly increased,which may be involved in the occurrence and development of gastric cancer.The combined detection of these three indicators can improve the accuracy of clinical diagnosis of gastric cancer,and has an important clinical application value.
作者
陈兰芬
CHEN Lanfen(Sichuan Provincial Hospital of Integrated Traditional Chinese and Western Medicine,Chengdu,Sichuan 610041,China)
出处
《检验医学与临床》
CAS
2019年第3期343-346,共4页
Laboratory Medicine and Clinic
