摘要
目的观察硫酸钙-链霉素缓释颗粒在兔体内的缓释效果及安全性评价。方法取大白兔48只,随机分为两组,每组24只。实验组在胫骨近端骨缺损置入的硫酸钙链霉素颗粒;对照组植入同样重量的链霉素粉剂。高效液相色谱仪(HPLC)测链霉素浓度。于术后1 d、2 d、3 d、1周、2周、4周、6周、8周、10周取静脉血1 mL待检;在孔周围取组织待检。术后2周、6周行肝肾抽血检查及肝、肾脏组织病理切片检查。术后1周、2周、4周、8周行植入部位X射线检查。结果实验组中链霉素在周围组织维持9周最低杀细菌浓度(minimum bactericidal concentration,MBC),植入10周尚处于最低抑菌浓度(minimal inhibitory concentration,MIC)。静脉血液中可维持8周MBC,植入9周尚处于MIC。对照组链霉素在周围组织的2周MBC,植入3周处于MIC;链霉素在血液的1周MBC,植入2周处于MIC。两组血液和组织的药物浓度差异均有统计学意义(P<0.05)。肝脏、肾脏功能生化检查、病理切片检查显示安全性良好,无毒性反应,两组肝脏、肾脏功能组间差异均无统计学意义(P>0.05)。X线及病理学检查显示实验组有着良好的成骨性。结论硫酸钙-链霉素颗粒在兔子体内缓慢释放,达到缓释效果,对肝肾组织无损伤。
Objective To observe the sustained release effect and safety evaluation of Calcium-streptomycin sulfate sustained-release granules in rabbits.Methods 48 rabbits were randomly divided into two groups,with 24 rabbits in each group.The experimental group was placed with calcium sulfate streptomycin granules in the proximal humerus bone defect.The control group was implanted with the same weight of streptomycin powder.The streptomycin concentration was measured by high performance liquid chromatography(HPLC).1 mL of venous blood was taken at 1 day,2 days,3 days,1 week,2 weeks,4 weeks,6 weeks,8 weeks,10 weeks after surgery for detection.The tissue was taken around the hole for examination.Liver and kidney blood tests and pathological biopsy of liver and kidney were performed at 2 weeks and 6 weaks after operation.X-ray examination of the implant site was performed at 1 week,2 weeks,4 weeks,and 8 weeks after surgery.Results Streptomycin in the experimental group maintained a minimum bactericidal concentration(MBC)for 9 weeks in the surrounding tissues,and was still at minimally inhibitory concentration(MIC)at 10 weeks after implantation.It could maintain a MBC for 8 weeks in venous blood and MIC at 9 weeks after implantation.Streptomycin in the control group maintained a minimum bactericidal concentration(MBC)for 2 weeks in the surrounding tissues,and was still at minimally inhibitory concentration(MIC)at 3 weeks after implantation.It could maintain a MBC for 1 week in venous blood and MIC at 2 weeks after implantation.There were significant differences in the drug concentrations of blood and tissue between the two groups(P<0.05).Liver and kidney function biochemical examination and pathological biopsy showed good safety and no toxicity.There was no significant difference between the two groups in liver and kidney function between two groups(P >0.05).X-ray and pathological examination showed that the experimental group had good osteogenicity.Conclusion Calcium-streptomycin sulfate particles are slowly released in rabbi
作者
陈勇忠
卫秀洋
翁少煌
刘永平
CHEN Yongzhong;WEI Xiuyang;WENG Shaohuang;LIU Yongping(Department of Orthopaedics,476 Hospital of Fuzhou General Hospital,Fuzhou 350002,China;College of Pharmacy,Fujian Medical University,Fuzhou 350002,China)
出处
《中国现代医生》
2018年第36期34-37,41,共5页
China Modern Doctor
基金
福建省自然科学基金资助项目(2015J01494)
关键词
硫酸钙
链霉素
缓释颗粒
抗结核
Calcium sulfate
Streptomycin
Sustained release granules
Anti-tuberculosis
