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应用mtDNA序列分析技术鉴定嗜尸性蝇类种属的研究进展 被引量:3

Research Progress about mtDNA Sequencing to Identify the Species of Sarcosaphagous Flies
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摘要 随着法医昆虫学的不断发展,应用嗜尸性昆虫的生长发育规律和尸体上昆虫演替规律推断死亡时间成为可能,但这要求快速、准确地鉴别嗜尸性昆虫的种属,然而,办案人员通常无法从形态上鉴别昆虫幼虫的种属,而对成虫的形态学种属鉴定知识也往往所知甚少,因此限制了相关技术在案件侦破中的广泛应用。自1994年sperling利用mtDNA序列分析方法进行种属鉴定以来,国内外许多研究者在此基础上进行了大量的尝试与探索,特别是近十几年,国内研究者在借鉴国外研究成果的基础上,在全国不同地域采集蝇类标本进行序列分析研究,在提取方法、基因片段种属鉴定效果、种属鉴定应用等方面取得了较大的研究成果。本文试图对国内近几年的研究进行总结,从而为后续研究提供参考。 Forensic entomology can provide clues for investigators to estimate the postmortem interval based on the growing and developing patterns of sarcosaphagous pupas showing their metamorphous evolutions on the corpses.However,only a few experts are able to master how to identify the species of sarcosaphagous pupas or flies using the morphology knowledge,thereby limiting the application of such entomologic capability into criminal detection.Fortunately,a large number of attempts both home and abroad have been explored to authenticate the sarcosaphagous pupas or flies with their mtDNA(mitochondrial DNA)sequencing since Sperling made such a kind of identification in 1994,having obtained plenty of achievements.Domestic researchers also acquired great progress on the aspects of collecting samples,evaluating the species identification through sequencing the relevant gene fragments of mtDNA,applying the method of species identification at the basis of gathering various flies specimens in different regions nationwide,and analyzing the flies’targeted mtDNA sequences.This review intends to make a summary about the latest domestic researches on this tendency.
作者 杨静波 刘龙 王江峰 YANG Jingbo;LIU Long;WANG Jiangfeng(Criminal Investigation Division of Hebei Provincial Public Security Department,Shijiazhuang 050000,China;Criminal Investigation Detachment of Bazhou Public Security Bureau,Bazhou 841000,Xinjiang,China;Department of Forensic Medicine,Soochow University,Suzhou 215000,Jiangsu,China)
出处 《刑事技术》 2019年第1期60-65,共6页 Forensic Science and Technology
基金 国家自然科学基金项目(No.30870332) 公安部应用创新计划资助项目(No.06011661)
关键词 法医昆虫学 MTDNA 嗜尸性蝇类 forensic entomology mtDNA sarcosaphagous flies
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