摘要
从石油污染土壤中分离得到1株石油降解菌1217,经细菌形态学、生理生化及16S rD NA序列分析初步鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。在温度5~65℃,pH 2~10,盐度0~9%的条件下菌株能很好生长,在以正十二烷、正十八烷、苯、甲苯、二甲苯和萘为唯一碳源的培养基中生长良好。在10℃和30℃条件下培养7 d,对原油的降解率分别为21.57%和15.15%。菌株产生的生物表面活性剂可以将表面张力从72.20 mN/m降至35.14 mN/m。利用特异性PCR扩增,在菌株中检测到烷烃单加氧酶、甲苯双加氧酶、联苯双加氧酶、芳香烃双加氧酶和氧化还原酶基因,并成功克隆出烷烃单加氧酶和芳烃双加氧酶基因,同相关基因比对分析,与铜绿假单胞菌PAO1的相应基因相似度分别为99.91%和99.22%。研究表明,菌株在生物修复和石油烃污染环境中具有潜在的应用价值。
One oil-degrading bacterial strain 1217 isolated from oil contaminated soil could degrade crude oil. It was identified and designated as Pseudomonas aeruginosaby morphology,physiology,biochemical and 16 S rD NA sequence analyses. The strain grew well at 5-65 ℃ with the initial pH of 2-10 and NaC l concentrations of 0%-9%. It grew well in the medium containing different organic substrates as sole carbon sources,such as n-dodecane,n-octadecane,benzene,toluene,xylene and naphthaline. The degrading rates for hydrocarbons were 21. 57% and 15. 15% when it was cultured in minimal medium containing crude oil and different chain alkanes at 30 ℃ and 10 ℃ for 7 days. The bacterium produced biosurfactants with the surface tension reduction from 72. 20 mN/m to 35. 14 mN/m. The oil-degrading related genes such as alkane monooxygenase,toluene dioxygenase,biphenyl dioxygenase,ring-hydroxylating dioxygenase and oxidoreductase genes were detected in Pseudomonas aeruginosa 1217. The alkane monooxygenase and ringhydroxylating dioxygenase genes were further cloned and analyzed. The sequence similarities of the two genes with those of Pseudomonas aeruginosa PAO1 were 99. 91% and 99. 22% respectively. The isolated strain exhibits great potential for the bioremediation of the hydrocarbons contaminated environments.
出处
《微生物学杂志》
CAS
CSCD
2017年第6期70-80,共11页
Journal of Microbiology
基金
The National Natural Science Foundation of China(31272694)
Technique Foundation of Lan Shi Equipment Engineering Research Institute Co.Ltd(J17-05)~~
关键词
石油降解
铜绿假单胞菌
鉴定
生物表面活性剂
降解基因
Oil-degradation
Pseudomonas aeruginosa
Identification
Biosurfactant
Degrading genes
