摘要
背景:如何提高钛合金种植体与骨组织的整合以及骨缺损修复效果是治疗骨缺损面临的主要问题。目的:观察3D打印骨科内植物结合低强度全身振动载荷对骨缺损修复和骨整合效果的影响。方法:选取24只雄性新西兰大白兔,建立股骨外侧髁缺损模型后随机分成观察组和对照组,每组12只。对照组采用3D打印技术制备多孔钛合金种植体处理,而观察组在对照组基础上使用低强度全身振动载荷处理。比较两组骨缺损模型兔采用不同方式处理后骨组织形态学参数、矿化沉积、骨整合情况及最大拔出力等。结果与结论:(1)观察组经全身振动刺激6,12周后,骨组织各参数优于对照组,其中骨小梁相对体积、骨小梁厚度及骨小梁数量显著高于对照组,骨小梁分离度和骨小梁相对表面积显著低于对照组(P <0.05);(2)观察组经全身振动刺激6,12周后,矿化面积比、矿化沉积率、骨形成率和最大拔出力均显著高于对照组,其中两组刺激12周的最大拔出力均显著高于6周(P <0.05);(3)结果证实,3D打印骨科内植物结合低强度全身振动载荷可有效促进骨组织的生长,加快骨缺损修复及提高骨整合效果。
BACKGROUND:How to improve the integration of titanium alloy implant with bones and repair results is an important issue in the treatment of bone defects.OBJECTIVE:To study the effects of three-dimensional printed orthopedic implants combined with low-intensity whole-body vibration load on bone defect repair and osseointegration.METHODS:Twenty-four male New Zealand white rabbits were selected for establishing the model of lateral condyle of femur,and randomly divided into observation and control groups(n=12 per group).The control group was treated with porous titanium alloy implants by three-dimensional printing technique,while the observation group was treated with low-intensity whole-body vibration load on the basis of the control group.The morphological parameters,mineralization,osseointegration,and maximal extraction force of rabbit bone tissue were compared between two groups.RESULTS AND CONCLUSION:(1)At 6 and 12 weeks of whole-body vibration stimulation,the parameters of bone tissue in the observation group were better than those in the control group.The relative volume of trabecular bone,trabecular thickness and trabecular bone in the observation group were significantly higher than those in the control group.The resolution and relative trabecular bone surface area in the observation group were significantly lower than those in the control group(P<0.05).(2)After 6 and 12 weeks of whole-body vibration stimulation in the observation group,the mineralization area ratio,mineralization deposition rate,bone formation rate and maximum extraction force were significantly higher than those in the control group,and the maximum extraction force in the two groups was significantly higher at 12 weeks than that at 6 weeks(P<0.05).(3)These results indicate that three-dimensional printing of orthopedic implants combined with low-intensity whole-body vibration load can effectively promote the growth of bone tissue,accelerate the repair of bone defects,and improve the effect of osseointegration.
作者
黎立
赵伊婷
何世凯
Li Li;Zhao Yiting;He Shikai(Orthopedic Center,Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University,Urumqi 830002,Xinjiang Uygur Autonomous Region,China;Department of Laboratory Medicine,the Sixth Affiliated Hospital of Xinjiang Medical University,Urumqi 830002,Xinjiang Uygur Autonomous Region,China;First Department of Orthopedics,People’s Hospital of Bortala Mongol Autonomous Prefecture,Bole 833400,Xinjiang Uygur Autonomous Region,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第12期1870-1874,共5页
Chinese Journal of Tissue Engineering Research
