摘要
采用平板对峙培养法,通过T2、J4细菌发酵上清液对苹果轮纹病菌丝生长的抑制作用实验,筛选了T2、J4细菌培养的最优培养基。结果表明:4号培养基(黄豆粉30 g、玉米粉30 g、NaCl 0.6 g)是T2细菌的最优培养基;1号培养基(蛋白胨6g、酵母浸膏3g)和2号培养基(蛋白胨3g、牛肉浸膏1.8 g、葡萄糖1.5 g)是J4细菌的最优培养基。
The optimal medium for T2 and J4 bacterial culture is screened by using the plate confrontation culture method to inhibit the growth of apple ringworm mycelium with T2 and J4 bacterial fermentation supernatants. The results show that: No. 4 medium (30g of soy flour, 30g of corn flour, 0.6g of Nacl) is the best medium for T2 bacteria; medium No. 1 (6g of peptone, 3g of yeast extract ) and No. 2 medium (3g of peptone, 1.8g of beef extract, 1.5g of glucose) are the optimal media for J4 bacteria.
作者
侯晓杰
梁魁景
HOU Xiaojie;LIANG Kuijing(College of Life Science, Hengshui University, Hengshui, Hebei 053000, China)
出处
《衡水学院学报》
2019年第1期10-12,共3页
Journal of Hengshui University
基金
河北省高等学校青年拔尖人才计划资助项目(BJ201406)
关键词
T2细菌
J4细菌
苹果轮纹病
最优培养基
T2 bacteria
J4 bacteria
the apple ring disease
the optimal medium
