摘要
背景:胎盘组织结构复杂,包括胎儿侧的羊膜、绒毛膜以及母体侧的蜕膜等。尽管一些文献已经报道这3种组织来源间充质干细胞具有一些共有的生物学特性,但是对于来自同一胎盘不同组织来源间充质干细胞是否存在不同的生物学特性,目前仍缺乏定量的研究分析。目的:探讨人胎盘不同组织来源间充质干细胞的生物学特性,包括分化潜能和免疫调节功能。方法:利用酶消化法从1例男婴胎盘组织分离羊膜来源间充质干细胞、绒毛膜来源间充质干细胞和蜕膜来源间充质干细胞。系统性研究这3种间充质干细胞的生物学特性,包括细胞形态、表面标志物表达、核型分析、成脂、成骨分化能力以及调节性T细胞的增殖能力。结果与结论:(1)3种细胞均呈成纤维细胞形态,均表达间充质干细胞的表面标志物:高表达CD73、CD90和CD105,低表达CD14、CD19、CD34、CD45和HLA-DR;(2)羊膜、绒毛膜间充质干细胞核型与胎儿相同,蜕膜间充质干细胞核型与母体相同;(3)3种间充质干细胞成脂分化能力存在显著性差异,依次为蜕膜间充质干细胞>绒毛膜间充质干细胞>羊膜间充质干细胞(P <0.05);相反,羊膜间充质干细胞的成骨分化能力明显高于蜕膜间充质干细胞(P <0.05);(4)羊膜间充质干细胞和绒毛膜间充质干细胞促进调节性T细胞增殖的能力显著高于蜕膜间充质干细胞;(5)结果表明,人胎盘羊膜、绒毛膜、蜕膜组织来源间充质干细胞具有不同的核型、成脂、成骨分化潜能和免疫调节能力,为不同种子细胞治疗相关疾病达到最佳效果提供了一定的参考。
BACKGROUND:The placental tissue structure is complex,including the amniotic membrane,chorion,and decidua from the mother.Mesenchymal stem cells derived from different tissues of the same placenta have been reported to have similar biological characteristics.To date,there is no study regarding quantitative comparison of differentiation potential and immunomodulatory function of mesenchymal stem cells derived from different tissues of human placenta.OBJECTIVE:To investigate the biological characteristics including differentiation potential and immunomodulatory function of mesenchymal stem cells derived from different tissues of human placenta.METHODS:The amnion-,chorion-,and decidua-derived mesenchymal stem cells were isolated from the placental tissue of a baby boy by enzymatic digestion method.The biological characteristics of these three kinds of mesenchymal stem cells were systematically investigated including cell morphology,immunophenotypes,karyotypeanalysis,adipogenic and osteogenic differentiation potential,and Treg cells proliferation capacity.RESULTS AND CONCLUSION:All three kinds of mesenchymal stem cells showed fibroblast-like morphology and expressed the surface markers of mesenchymal stem cells with high expressions of CD73,CD90 and CD105,as well as low expressions of CD14,CD19,CD34,CD45 and HLA-DR.The karyotypes of the amnion-and chorion-derived mesenchymal stem cells were the same as the fetus,and decidua-derived mesenchymal stem cells had the same karyotype as the mother.There were significant differences in adipogenic differentiation capacity between three kinds of mesenchymal stem cells(amnion-derived mesenchymal stem cells>chorion-derived mesenchymal stem cells>decidua-derived mesenchymal stem cells;P<0.05).In contrast,the osteogenic differentiation capacity of amnion-derived mesenchymal stem cells was remarkably higher than that of decidua-derived mesenchymal stem cells(P<0.05).The amnion-and chorion-derived mesenchymal stem cells had the higher potential of Treg cell proliferation induction th
作者
王留娣
刘威
谢园园
高天芸
黄霏霏
王斌
Wang Liudi;Liu Wei;Xie Yuanyuan;Gao Tianyun;Huang Feifei;Wang Bin(Department of Clinical Stem Cell Research,Nanjing Drum Tower Hospital,Nanjing University Medical School,Nanjing 210008,Jiangsu Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第9期1377-1383,共7页
Chinese Journal of Tissue Engineering Research
基金
科技部2017年重大研发计划干细胞专项(2017YFA0104300)
项目负责人:顾宁
国家自然科学基金面上项目(81571213)
项目负责人:王斌
江苏省第十三批"六大人才高峰"项目(WSN-155)
项目负责人:王斌
南京市"十三五"规划重点项目(ZDX16005)
项目负责人:王斌
南京市"十三五"青年人才项目(第一层次)(QRX17006)
项目负责人:王斌~~
