摘要
为了确定石河子地区规模化羊场出现呼吸道症状死亡羊的细菌性病原,采用常规细菌分离鉴定方法、细菌16SrRNA序列分析以及多杀性巴氏杆菌特异性基因kmt,从病变肺组织分离鉴定细菌,利用5个荚膜血清型特异性基因确定其血清型,扩增分离株的16个毒力相关基因,分析分离菌致病性和对常用抗菌药物的耐药性。结果表明,从病羊的病变肺组织中分离鉴定到一株血清D型多杀性巴氏杆菌,具有较强的致病性;携带8个毒力相关基因,其片段序列与NCBI上己公布的多杀性巴氏杆菌参考株同源性在99%以上;分离株对青霉素、林可霉素、庆大霉素、复方新诺明耐药,对其他23种药物敏感。
In order to determine the cause of the sheep died of respiratory infection from a sheep farm in Shihezi,bacterial pathogens were isolated and identified by conventional methods and molecular biological methods based on 16S rRNA special sequence and Pm specific gene kmt sequence analysis.Serotype of the isolate was determined with the primers from 5 capsular serotype-specific genes,16 virulence-associated genes were detected,pathogenicity and drug sensitivity of the isolate were analysed.The results showed that the isolate was a serotype D Pm with strong pathogenicity,which carried 8 virulence-associated genes and the amplified fragment homology of the corresponding sequence in NCBI is more than 99%.The isolate was resistant to penicillin,lincomycin,gentamicin,cotrimoxazole.
作者
王蕾
邬琴
柴迎锦
梅朱园
郭强强
黄新
韩猛立
张星星
周霞
钟发刚
WANG Lei;WU Qin;CHAI Ying-jin;MEI Zhu-yuan;GUO Qiang-qiang;HUANG Xin;HAN Meng-li;ZHANG Xing-xing;ZHOU Xia;ZHONG Fa-gang(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang,832003,China;State Key Laboratory of Genetic Improvementand Healthy Breeding of Sheep,Xinjiang Academy of Agricultural Sciences/Provincial Department,Shihezi,Xinjiang,832000,China)
出处
《动物医学进展》
北大核心
2019年第1期25-30,共6页
Progress In Veterinary Medicine
基金
省部共建绵羊遗传改良与健康养殖国家重点实验室优秀中青年人才培养引导计划专项资助项目(SKLGIH2016A02)
2017年度省部共建绵羊遗传改良与健康养殖国家重点实验室开放课题(MYSKLKF 201804)
2017年国家级创新创业训练计划项目(2017107590)
兵团现代农业科技攻关与成果转化计划项目(2016AC018)
关键词
羊呼吸道疾病
D型多杀性巴氏杆菌
分离鉴定
毒力基因
sheep respiratory disease
Pasteurella multocida serotype D
isolation and identification
virulence gene
