摘要
为了明确土霉素废水的生物毒性效应,采用暴露实验方法,研究了土霉素废水对斑马鱼的毒性效应。结果显示:对斑马鱼而言,土霉素废水属于低毒物质,斑马鱼暴露3～9d时,其SOD活性和POD活性受到不同程度的抑制,12d时受到显著诱导(0.01<P<0.05),20%体积浓度组SOD活性达到最大值(194.05U/mg prot),60%体积浓度组POD达到最大值(2.72U/mg prot),15d时均恢复至对照组水平;SOD活性与POD活性具有一定相关性,各体积浓度组MDA含量整体呈"Λ"型变化趋势,80%体积浓度组在第15d达到最大值(5.41nmol/mg prot);斑马鱼肌肉组织中γ-H2AX含量呈现升高趋势,并在第15d时40%体积浓度组表现为极显著性(400.02ng/L,P<0.01),为对照组的1.57倍,表明斑马鱼肌肉细胞中的DNA受到一定程度的损伤。因此,斑马鱼对土霉素废水的氧化应激可能是造成DNA损伤的机制之一,对其生物毒性进行探讨,可为进一步研究土霉素在水环境中的生态毒性效应提供科学根据。
In order to clarify the biotoxic effects of oxytetracycline wastewater,the toxicity effects of oxytetracycline wastewater on zebrafish are studied by exposure test.The results show that oxytetracycline wastewater is a low toxic substance to zebrafish.The SOD activity and POD activity of zebrafish are differently inhibited at 3～9d.At 12 d,SOD activity and POD activity are significantly induced(0.01<P<0.05).The SOD activity of 20%volume concentration group reaches the maximum(194.05U/mg prot),and the POD of 60% volume concentration group reaches the maximum value(2.72U/mg prot).The SOD activity and POD activity almost returns to control values at 15 d.In addition,there is significant correlation between SOD activity and POD activity in research.The MDA content shows a "Λ" trend in each volume concentration group,and the 80%volume concentration group reaches the maximum value(5.41nmol/mg prot)at 15 d.The content ofγ-H2 AX shows a risingtrend in zebrafish muscle tissue.At 15 d,the 40%volume concentration group shows extremely significant(400.02ng/L,P<0.01),which is 1.57 times that of the control group.The DNA of zebrafish muscle cells has been damaged in a certain degree.This study shows that oxytetracycline wastewater is a low toxic substance to zebrafish,and the oxidative stress of zebrafish to oxytetracycline wastewater may be one of the mechanisms causing DNA damage.The biological toxicity of oxytetracycline can provide a scientific basis for further study of the ecotoxicity effect of oxytetracycline in water environment.
作者
高问
武鹏鹏
王雅学
沈洪艳
GAO Wen;WU Pengpeng;WANG Yaxue;SHEN Hongyan(School of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018,China;Laboratory of Hebei Province for Medical Molecular Chemistry,Shijiazhuang,Hebei 050018,China)
出处
《河北科技大学学报》
CAS
2019年第1期38-44,共7页
Journal of Hebei University of Science and Technology
基金
国家自然科学基金(41373096)
国家环保公益性行业科研专项课题(201509041-05)
河北省自然科学基金(B2014208068)
河北省药用分子化学实验室开放基金
河北省环保厅公益课题
河北省重点学科建设基金项目
关键词
环境毒理学
土霉素废水
斑马鱼
超氧化物歧化酶
丙二醛
DNA损伤
environmental toxicology
oxytetracycline wastewater
zebrafish
superoxide dismutase
malonaldehyde
DNA damage
