摘要
通过超声波辅助提取常春油麻藤总黄酮,在单因素试验基础上,利用响应面法优化常春油麻藤总黄酮提取工艺,并对羟基自由基清除能力和牛血清白蛋白结合效应进行评价。结果表明:当料液比1∶25(g·mL-1)、甲醇体积分数60%、超声时间20min、超声功率420W,总黄酮含量预测值为3.64mg·g-1,验证值为3.58mg·g-1,与理论值的相对误差为1.64%,表明该优化方法合理可行。常春油麻藤总黄酮提取液对羟基自由基最大清除率可达85.35%;对牛血清白蛋白具有一定的荧光淬灭作用,淬灭方程为Y=-233.9ln(X)+25.665,R2=0.9906。
An ultrasonic extraction of flavonoids from Mucunae sempervirens was optimized using a single factor response surface methodology.The content,hydroxyl radical scavenging capacity and bovine serum albumin(BSA)combining ability of the resulting extract were determined for the evaluation.The results showed the optimized conditions to include a methanol volume fraction of 60% and a solid-liquid ratio of 1∶25(g·mL-1)with 420 W ultrasound applied for 20 min to reach a total extracted flavonoid content of 3.64mg·g-1.In comparison to the verification content of 3.58mg·g-1,the difference between the theoretical and experimental values was 1.64%indicating that a reasonable and feasible result had been reached.The hydroxyl radical scavenging rate of the extract was 85.35%,and the fluorescence quenching effect of the extract on the BSA combination test followed the exponential function of Y=-233.9ln(X)+ 25.665(R2=0.9906).
作者
林志銮
金晓怀
张传海
李宝银
华伟平
LIN Zhi-luan;JIN Xiao-huai;ZHANG Chuan-hai;LI Bao-yin;HUA Wei-ping(Wuyi University Analysis and Testing Center,Nanping,Fujian 354300,China;Yushan Health Management College,Wuyi University,Nanping,Fujian 354300,China;Fujian Provincial Key Laboratory of Eco-Industrial Green Technology,Nanping,Fujian 354300,China;Fujian Normal University,Fuzhou,Fujian 350108,China)
出处
《福建农业学报》
CAS
北大核心
2018年第11期1212-1218,共7页
Fujian Journal of Agricultural Sciences
基金
福建省教育厅科技项目(JA15521)
福建省生态产业绿色技术重点实验室项目(WYKF2017-8)
福建省科技厅中央引导地方科技发展专项(2018L3018)
关键词
常春油麻藤
总黄酮
响应面法
羟基自由基
淬灭
Mucunae sempervirens
total flavonoids
response surface methodology
hydroxyl radical scavenging
quenching
