摘要
目的:优选了海南黎药冰糖草茎叶中总黄酮成分提取工艺。方法:采用L_(16)(4~5)正交试验,以冰糖草茎叶中总黄酮得率为考察指标,考察乙醇浓度、超声时间、超声温度、超声功率和液料比5个因素对总黄酮得率的影响。结果:海南黎药冰糖草茎叶中总黄酮提取的最佳工艺为80%乙醇浓度、80min超声时间、50℃超声温度、250W超声功率、30∶1ml/g液料比,此条件下总黄酮得率达25.36mg/g。结论:应用该工艺提取黎药冰糖草茎叶中总黄酮,总黄酮得率较高,方便可行,为黎药冰糖草中总黄酮提取工艺的确定提供实验依据。
Objection:To optimize the extraction technology of total flavoniods in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L.Methods:By orthogonal design L16(45),we changed the ethanol concentration,ultrasonic time,ultrasonic temperature,ultrasonic power and ratio of liquid-to-solid in order to obtain highest extraction rate of total flavonoids.Results:The best technological parameters for extraction of total flavonoids in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L.are as follows:ethanol concentration of 80%,ultrasonic time of 80 min,ultrasonic temperature of 50℃,ultrasonic power of 250W,liquid solid ratio of 30:1ml/g.Under such condition,the yields of total flavonoids were 25.36mg/g.With high extraction yields of total flavonoids,this technologogy is simple and feasible.Conclusion:This study provides experimental basis for extraction of total flavonoids from in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L.
作者
鱼清雅
郭从嘉
毕进
张思程
符亮明
侯杰
陈丽珍
YU Qingya;GUO Congjia;BI Jin(School of Pharmaceutical Science, Hainan Medical College ,Haikou 571199)
出处
《陕西中医》
2019年第1期131-134,共4页
Shaanxi Journal of Traditional Chinese Medicine
基金
国家级大学生创新创业创新训练计划项目(201711810028)
海南医学院大学生创新训练项目(HYXC2016030)
海南医学院科研培育基金(HY2015-2)
关键词
海南黎药
冰糖草
总黄酮
正交试验
提取工艺
Hainan Li Folk medicine
Scoparia dulcis L
Total flavonoids
Orthogonal test
Extraction technology
