摘要
目的:筛选甘蔗叶具有抗肝损伤作用的活性部位。方法:以80%乙醇提取甘蔗叶得到乙醇提取物浸膏,用水分散后依次用石油醚、乙酸乙酯、正丁醇进行萃取,得到相应极性部位萃取物,剩余为水部位萃取物。将108只小鼠随机分为空白A组[0.5%羧甲基纤维素钠(CMC-Na)与3.5%聚山梨酯80溶液]、空白B组(纯净水)、模型组(纯净水)、联苯双酯组(阳性对照,0.2 g/kg)、甘蔗叶乙醇提取物组和甘蔗叶不同极性部位(石油醚、乙酸乙酯、正丁醇、水)组(以生药量计给药量均为22.92 g/kg,以0.5%CMC-Na与3.5%聚山梨酯80溶液为溶剂),每组12只。每天灌胃给药1次,连续给药12 d。末次给药1 h后,除空白A、B组外,其余各组小鼠均腹腔注射0.15%四氯化碳(CCl4)-花生油(0.1 mL/10 g)溶液复制急性肝损伤模型。16 h后,观察小鼠一般情况,并检测其血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)含量,观察其肝组织病理形态学变化并进行Ishak评分。结果:与空白B组比较,空白A组小鼠各指标均无明显差异(P>0.05)。与空白A组比较,模型组小鼠毛发稀疏,体型消瘦,活动稍显迟缓;血清中ALT、AST含量显著升高(P<0.01);肝小叶结构破坏严重,肝索和肝窦结构不清晰,肝索排列紊乱,Ishak评分显著升高(P<0.01)。与模型组比较,各给药组小鼠一般情况均有所好转;联苯双酯组、甘蔗叶乙醇提取物组和甘蔗叶乙酸乙酯部位组小鼠血清中ALT、AST含量显著降低(P<0.05或P<0.01),且肝组织的病理损伤也明显减轻,Ishak评分显著降低(P<0.05或P<0.01)。结论:甘蔗叶乙醇提取物的乙酸乙酯部位为其具有抗CCl4致小鼠急性肝损伤作用的活性部位。
OBJECTIVE:To screen the active part of Saccharum officinarum leaves against liver injury.METHODS:The S.officinarum leaves were extracted with 80%ethanol to obtain ethanol extract,after dispersed with water,ethanol extract was respectively extracted with petroleum ether,ethyl acetate and n-butanol to obtain corresponding polar parts.The residual part was water part.Totally 108 mice were randomly divided into blank group A(0.5%CMC-Na and 3.5%polysorbate 80 solution),blank group B(purified water),model group(purified water),biphenyl diester group(positive control,0.2 g/kg),S.officinarum leaves ethanol extract group,different polar parts of S.officinarum leaves group(petroleum ether,ethyl acetate,n-butanol,water,22.92 g/kg crude drug,0.5%CMC-Na and 3.5%polysorbate 80 solution as solvent),with 12 mice in each group.They were given relevant medicine intragastrically once a day,for consecutive 12 d.1 h after last medication,except for blank group A and blank group B,mice in other groups were given 0.15%CCl4 peanut oil(0.1 mL/10 g)solution to induce acute liver injury model.16 h later,general information of mice was observed,and the serum contents of ALT and AST were determined.The liver histopathological changes were observed and the Ishak scores were scored.RESULTS:Compared with blank group B,each index of blank group A had no significant difference(P>0.05).Compared with blank group A,model group had sparse hair and slow movement,and was emaciated.Serum contents of ALT and AST were increased significantly(P<0.01).The structure of hepatic lobule was severely damaged;the structure of hepatic cord and sinus was not clear;the arrangement of hepatic cord was disordered,and the Ishak score was significantly increased(P<0.01).Compared with model group,general information of mice was improved in administration groups.Serum contents of ALT and AST were decreased in biphenyl diester group,S.officinarum leaf ethanol extract group and S.officinarum leaf ethyl acetate group(P<0.05 or P<0.01).The pathological damage of liver tissue
作者
郝二伟
李扬
赵媛媛
邓家刚
侯小涛
HAO Erwei;LI Yang;ZHAO Yuanyuan;DENG Jiagang;HOU Xiaotao(Guangxi Key Lab of Efficacy Study on Chinese Materia Medica,Guangxi University of TCM,Nanning 530200,China;Collaborative Innovation Center of Functional Ingredients Study of Agricultural Residues,Guangxi University of TCM,Nanning 530200,China;College of Pharmacy,Guangxi University of TCM,Nanning 530200,China)
出处
《中国药房》
CAS
北大核心
2019年第1期84-88,共5页
China Pharmacy
基金
国家自然科学基金地区科学基金项目(No.81360655)
广西科技计划项目(No.桂科AD17195025)
广西重点实验室建设项目(No.17-259-20)
关键词
甘蔗叶
乙醇提取物
急性肝损伤
活性部位
小鼠
Saccharum officinarum leaves
Ethanol extract
Acute liver injury
Active part
Mice
