摘要
目的探讨香椿叶提取物(TSLE)对高脂诱导大鼠视网膜损伤的保护作用及其作用机制。方法选取清洁级雄性SD大鼠24只(48只眼),随机分为正常组(N组)(8只)和模型组(16只)。模型组胃饲高脂饲料(10 ml/kg),正常组给予相同剂量生理盐水。8周后测两组空腹血脂,确定模型复制成功后,将模型组随机分为高脂饮食组(HF组)和高脂饮食+香椿叶提取物组(HF+TSLE组),每组8只。HF+TSLE组给予TSLE(1 g/kg)水溶液灌胃喂养,N组和HF组给予等量生理盐水。TSLE干预4周后,所有大鼠进行闪光视网膜电图(FERG)检查、血脂及肝脏指数检测;取各组动物眼行HE染色,观察视网膜组织的形态学改变;免疫组织化学和Western blotting检测视网膜组织中IL-6、TNF-α、NF-κB p65及Caspase-3蛋白的表达水平。结果 3组大鼠的TC、TG、LDL-C及HDL-C水平比较,差异有统计学意义(P <0.05)。组间两两比较显示,HF组血脂含量与N组比较,差异有统计学意义(P <0.05),HF+TSLE组血脂含量与N组比较,差异无统计学意义(P>0.05);HF+TSLE组血脂含量与HF组比较,差异有统计学意义(P <0.05)。3组大鼠a波潜伏期比较,差异有统计学意义(P <0.05);3组b波潜伏期、a波振幅及b波振幅比较,差异无统计学意义(P>0.05);其中N组、HF+TSLE组a波潜伏期分别与HF组比较,差异有统计学意义(P <0.05);3组大鼠视网膜厚度比较差异有统计学意义(P <0.05),其中HF组与N组、HF+TSLE组与HF组比较差异有统计学意义(P <0.05),HF+TSLE组与N组比较差异无统计学意义(P>0.05)。3组IL-6、TNF-α、NF-κB p65、Caspase-3表达水平比较,差异有统计学意义(P <0.05),HF组蛋白表达水平较N组提高;TSLE干预后,能够有效地下调IL-6、TNF-α、NF-κB p65、Caspase-3的表达水平。结论 TSLE能够抑制高脂引起的视网膜损伤;TSLE通过抑制IL-6、TNF-α、NF-κB p65、Caspase-3的表达来对抗组织凋亡。
Objective To investigate the protective effect of TSLE on high fat induced retinal injury in rats and its mechanism.Methods A total of 24 male SD rats were randomly divided into normal group (N group),high fate (HF) group and HF + Toona Sinensis Leaves Extract (TSLE) group,8 for each group.Rats in HF group and TSLE group were fed with high fat diet (10 ml/kg),and rats in normal group was given the same dose of normal saline.After 8 weeks,fasting blood lipids were measured in two groups.Rats in HF+TSLE group were fed with TSLE water solution (1 g/kg),rats in N group and HF group were given the same amount of normal saline.After TSLE intervention for 4 weeks,all rats were examined by flash electroretinogram (FERG).Blood levels of lipid and liver function were recorded.Morphological changes of retinal tissue were evaluated by HE staining.Immunohistochemistry and Western blot were performed for expression of IL-6,TNF-α,NF-κB p65,and caspase-3 protein in retina tissue.Results Serum levels of lipid levels including TC,TG,LDL-C and HDL-C were significantly different among groups;those in HF group were increased significantly when compared with those in N group (P<0.05);those levels between N group and HF+TSLE group were not significantly different (P>0.05) but significantly different between HF group and HF+TSLE group (P<0.05).α wave latency among groups were statistically different (P<0.05).No obvious differences in β wave incubation period,β wave,and α wave were observed among groups (P>0.05).There was difference in α wave incubation period between HF group and N group or HF+TSLE group.Retinal thickness in HF group was significantly increased compared with N group (P<0.05),which was diminished with treatment of TSLE (P<0.05).The results of immunohistochemistry and Western blotting showed that the levels of IL-6,Caspase-3,TNF-α,and NF-κBp65 in HF group were significantly enhanced (P<0.05),which were decreased by treatment with TSLE (P<0.05).Conclusion TSLE protects high fat induced retinal damage through inhib
作者
张园园
于利
Yuan-yuan Zhang;Li Yu(Graduate School,Jinzhou Medical University,Jinzhou,Liaoning 121001,China;Department of Physiology,Jinzhou Medical University,Jinzhou,Liaoning 121001,China)
出处
《中国现代医学杂志》
CAS
2019年第1期7-14,共8页
China Journal of Modern Medicine
基金
辽宁省自然科学基金(No:20170540399)
