摘要
目的探讨血脂异常载脂蛋白E(apolipoprotein E,ApoE)基因缺失(ApoE-/-)小鼠视网膜色素上皮(retinal pigment ep-ithelium,RPE)细胞胞浆内黑色素和脂褐素的改变。方法将24只2个月龄ApoE-/-小鼠随机分为高脂组和普食组,每组12只,另用12只2个月龄C57BL小鼠作为普食组,喂养4个月后检测其血浆总胆固醇(total plasma cholesterol,TC)、低密度脂蛋白(low density lipoprotein,LDL)、甘油三酯(plasma triglyceride,TG)含量,Masson三色、银氨液浸染法和醛品红法染色后光镜下观察视网膜光感受器细胞(photoreceptor cell,PRC)、RPE细胞、Bruch膜形态结构,半定量检测视网膜PRC数、RPE细胞数、Bruch膜厚度、RPE细胞胞浆内黑色素和脂褐素含量,采用逐步引入-剔除法多元线性回归分析RPE细胞数与TC、LDL、TG含量的相关性及RPE细胞胞浆内黑色素、脂褐素含量与PRC数、RPE细胞数、Bruch膜厚度的相关性。结果ApoE-/-高脂组、ApoE-/-普食组、C57BL普食组小鼠血浆TC的含量分别为(13.262±0.458)mmol·L-1、(9.722±0.412)mmol·L-1、(5.150±0.308)mmol·L-1,LDL的含量分别为(2.030±0.186)mmol·L-1、(1.047±0.158)mmol·L-1、(0.427±0.080)mmol·L-1,TG的含量分别为(1.233±0.199)mmol·L-1、(0.777±0.161)mmol·L-1、(0.530±0.168)mmol·L-1,PRC细胞核面积分别为(20135.000±1473.272)μm2、(23208.000±716.913)μm2、(26952.000±2449.104)μm2,RPE细胞数分别为8.900±1.148、10.200±0.835、13.800±1.016,Bruch膜厚度分别为(0.982±0.071)μm、(0.870±0.042)μm、(0.594±0.031)μm。ApoE-/-高脂组和普食组小鼠TC、LDL、TG含量较C57BL普食明显增高(P<0.05或0.001)。光镜下观察发现:ApoE-/-高脂组和ApoE-/-普食组小鼠视网膜PRC、RPE细胞排列紊乱、萎缩,RPE细胞内黑色素分布稀疏、面积小,脂褐素分布密集、范围广,Bruch膜粗细不等,胶原分叉,部分小鼠视网膜下可见玻璃膜疣形成;半定量结果显示,ApoE-/-高脂组和ApoE-/-普食组小鼠视网膜PRC细胞核面积、RPE细�
Objective To explore the changes of melanin and lipofuscin in retinal pigment epithelium intracytoplasm of mice with dyslipidemia and apolipoprotein Edeficiency （ApoE-/-）. Methods A total of 24 two-month-old ApoE-/- mice were equally and randomly divided into high fat diet group and normal diet group ,and another 12 two-month-old C57BL mice were chosen as normal diet control group. After they were all fed for 4 months,levels of total plasma cholesterol（TC） ,low density lipoprotein（LDL） and plasma trigiyceride（TG） were detected ,morphological characteristics of retinal photoreceptor cell （ PRC ）, retinal pigment epithelium （ RPE ） and Bruch mem- brane were observed by fight microscope after the staining of Masson trichrism staining,fontana staining and aldehydo-magenta staining,and amounts of retinal PRC and RPE, the contents of melanin and lipofuscin in RPE cells and the thickness of Bruch＇ s membrane were examined by semi-quantitative histopathology, the relevance between RPE and TC,LDL,and TG content was analyzed,as well as the relevance between the contents of melanin and lipofuscin in RPE cells and the number of PRC, RPE cells, and the thickness of Bruch＇ s membrane by gradually introduced-excluding method of multiple linear regression. Results The levels of TC were （ 13. 252 ± 0. 458 ） mmol·L-1 , （9. 722 ±0.412）mmol·L-1 and （5. 150 ±0. 308）mmol·L-1 ,levels of LDL were （2. 030 ± 0. 186）mmol·L-1,（1.047 ±0. 158）mmol·L-1 and （0.427 ±0.080）mmol·L-1 ,levels of TG were （1.233±0.199）mol·L-1, （ 0. 777 ± 0.151）mol·L-1 and （0.530±0.158）mml·L-1 ,nucleus areas of PRC were （20 135. 000 ± 1473.272）μm2 , （20 208.000 ±716. 913） μm2 and （26 952.000 ±2449. 104） μm2 and numbers of RPE cells were 8. 900 ± 1. 481,10. 200 ± 0. 835 and 13.800 ± 1. 016, thickness of Bruch＇ s membrane were （ 0. 982 ± 0. 071 ） μm, （0. 870 ± 0. 042 ） μm and （0. 594 ±0.031 ） μm in blood plasma of mice in ApoE-/- high fat diet group,
出处
《眼科新进展》
CAS
北大核心
2013年第7期605-609,共5页
Recent Advances in Ophthalmology
基金
国家自然科学基金资助(编号:30973774)~~
