摘要
为快速、准确检测烟草中高级脂肪酸含量,建立了测定烟草中7种高级脂肪酸的液相色谱-串联质谱(LC-MS/MS)方法。样品经KOH的甲醇溶液皂化,二氯甲烷萃取后,十七烷酸作内标直接进样LC-MS/MS分析定量。结果表明各高级脂肪酸峰面积与内标峰面积的比值和其浓度与内标浓度之比的线性关系良好(R2≥0.9818),加标回收率在87.5%～109%之间,平均相对标准偏差(RSD)在1.1%～4.7%之间,方法的检出限(LOD)和定量限(LOQ)分别为0.0004～0.007 mg/g和0.001～0.02 mg/g。该方法适用于烟草7种高级脂肪酸的快速测定,具有简单、快速、灵敏等特点。
A method for determining 7 high fatty acids in tobacco by high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)was developed.Sample was saponified with KOH-methanol solution,extracted with dichloromethane,and then injected directly into sample for LC-MS/MS analysis using heptadecanoic acid as internal standard for quantitative analysis.Results showed that the linear regression lines for peak area ratios(analyte/internal standard)plotted against the concentration ratios(analyte/internal standard)fitted well(R^2≥0.9818)for high fatty acids.The recoveries of standard addition ranged from 87.5%to 109%with relative standard deviation(RSD)of 1.1%~4.7%.The limit of detection was between 0.0004 and 0.007 mg/g,and that of quantification was between 0.001 and 0.02 mg/g.This method is simple,accurate and suitable for the determination of 7 high fatty acids in tobacco.
作者
马丽伊
徐志强
田振峰
赵爱侠
淦五二
MA Liyi;XU Zhiqiang;TIAN Zhenfeng;ZHAO Aixia;GAN Wuer(Department of Chemistry,University of Science and Technology of China,Hefei 230026,China;Technology Center,China Tobacco Anhui Industrial Co.,Ltd. Hefei 230088,China)
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2018年第6期1-8,共8页
Acta Tabacaria Sinica
基金
安徽中烟工业有限责任公司联合实验室科技项目(2015124)
