摘要
为快速检测分析烟叶中游离脂肪酸含量,建立了利用超高效合相色谱-质谱法(UPC2/MS)快速分析8种烟叶中棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸5种脂肪酸的方法,并利用该法鉴别了32种游离脂肪酸。结果表明,采用ACQUITY UPC2色谱系统和Xevo TQS质谱仪,以HSS C18色谱柱(SB 1.8μm,2.1 mm×150 mm)为分析柱,以CO_(2)和含0.1%甲酸的甲醇溶液为流动相进行梯度洗脱,5种脂肪酸在2.2~72.2 ng/mL具有较好的线性关系(r>0.999),检出限(S/N≥3)为0.06~0.13 ng/mL,定量限(S/N≥10)为0.19~0.41 ng/mL,精密度RSD为5.2%~8.0%,稳定性RSD为4.3%~7.5%,重复性试验RSD为3.0%~6.7%,不同添加水平下的平均回收率为95.8%~97.2%;8种烟叶中棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸的含量在0.12~1.21 mg/g。该方法分析速度快、灵敏度高、选择性强,且样品前处理简单,无需衍生化,适用于烟叶中游离脂肪酸的测定。
In order to quickly detect and analyze the content of free fatty acids in tobacco leaves,Ultraperformance convergence chromatography mass spectrometry(UPC2/MS)was established for the rapid analysis of 5 kinds of fatty acids,palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid,in 8 kinds of tobacco leaves,and identification of 32 kinds of free fatty acids.The results showed that HSS C18 chromatographic column(SB 1.8μm,2.1 mm×150 mm)was used as the analytical column,CO_(2) and 0.1%formic acid solution of methanol was used as the mobile phase to gradient elution by ACQUITY UPC2 chromatographic system and Xevo TQS mass spectrometer.The 5 kinds of fatty acids had a good linear relationship within the range of 2.2~72.2 ng/mL(r>0.999);the detection limit(S/N≥3)was 0.06~0.13 ng/mL;the quantitative limit(S/N≥10)was 0.19~0.41 ng/mL;the relative standard deviation(RSD)of precision was 5.2%~8.0%;the relative standard deviation(RSD)of stability was 4.3%~7.5%;the relative standard deviation(RSD)of repeatability test was 3.0%~6.7%;the average recovery rate was 95.8%~97.2%at different addition levels.The contents of palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid in 8 tobacco leaves ranged from 0.12 mg/g to 1.21 mg/g.The method is rapid,sensitive,selective and simple in sample pretreatment without derivation.It is applicable for the determination of free fatty acids in tobacco leaves.
作者
喻世涛
陶永峰
姚建武
魏刚
高颂
杨鑫
唐向兵
YU Shi-tao;TAO Yong-feng;YAO Jian-wu;WEI Gang;GAO Song;YANG Xin;TANG Xiang-bing(China Tobacco Hubei Industrial CO.,Ltd.,Wuhan 430040,China;Hubei Xinye Reconstituted Tobacco Development Co.,Ltd.,Wuhan 430056,China;Applied Technology Research of Reconstituted Tobacco Hubei Province Key Laboratory,Wuhan 430040,China)
出处
《湖北农业科学》
2021年第5期108-112,共5页
Hubei Agricultural Sciences
基金
湖北中烟工业有限责任公司资助项目(2018B032BP03)。
