摘要
目的观察针刺长强穴对自闭症模型大鼠学习记忆功能、海马区β-catenin和GSK-3β蛋白表达的影响。方法选健康成年Wistar雄性及雌性大鼠各10只,进行雌、雄合笼,随机分为2组,一组在怀孕12 d时给予母鼠腹腔注射丙戊酸钠,其产下的仔鼠随机分为非针刺对照组10只、非穴治疗对照组10只、针刺"长强"穴组9只。一组产下空白对照组。治疗结束后行水迷宫实验检测及免疫组化技术检测。结果针刺长强穴组及空白对照组与非针刺对照模型组相比,其平均逃避潜伏期明显缩短(P<0.05),其海马区β-catenin蛋白阳性细胞率明显下调(P<0.05),其海马区GSK-3β蛋白阳性细胞率明显上调(P<0.05)。结论针刺"长强"穴可通过调节Wnt通路上的β-catenin和GSK-3β蛋白产生影响进而提高自闭症模型大鼠学习记忆能力。
Objective To study the effet of acupuncture at Changqiang(GV1)on the learning and memory function in rat models of autism as well as on theβ-catenin and GSK-3βprotein expression of hippocampus region.Methods Choosing 10 healthy adult Wistar male rats and female rats,they were randomly divided into 2 groups.One group was injected with valproate after 12 days of pregnancy,and the birth of rats were randomly divided into a non-acupoint group,a model group,an electroacupuncture at Changqiang group.Another group gave birth to a blank control group.After treatment,the water maze test and immunohistochemical test were performed.Results Compared with the non-acupoint group,the average escape latency was significantly shortened(P<0.05)by a blank control group and an electroacupuncture at Changqiang group,the hippocampusβ-catenin protein positive cells rate significantly lowered(P<0.05),and the hippocampus GSK-3βprotein positive cells rate significantly raised(P<0.05).Conclusion The acupuncture at Changqiang(GV1)can be regulated by Wnt pathway on theβ-catenin and GSK-3βprotein protein and improve learning and memory ability in rat models of autism.
作者
俞萍
郑昌岳
李凤
蒋剑文
YU Ping;ZHENG Chanyue;LI Feng;JIANG Jianwen(Physiatry Department,Fujian Province Governmental Hospital,Fujian province,Fuzhou 350001,China)
出处
《中国中医药现代远程教育》
2019年第1期111-113,共3页
Chinese Medicine Modern Distance Education of China
基金
福建省卫生计生委青年科研课题项目【No.2017-1-35】
关键词
自闭症
长强穴
海马
WNT通路
动物实验
蛋白表达
郁证
autism
Changqiang(GV1)
hippocampus
Wnt pathway
animal experiment
protein expression
melancholia
