摘要
为高效筛选氮高效油菜资源,建立甘蓝型油菜苗期氮效率评价方法,以162份油菜育种品系为材料,设置正常氮供给(氮浓度为9 500μmol/L)和低氮胁迫(氮浓度为237. 5μmol/L)两个氮水平处理,采用营养液培养方法,研究不同氮水平下油菜苗期生物学性状及氮累积量,并对所有品系进行氮效率评价和基因型差异分类。两年试验结果显示,油菜生物量、主根长、侧根长、根冠比、氮累积量及氮吸收和利用效率在不同氮水平处理间差异极显著。不同氮水平下,油菜苗期生物量与氮累积量、氮利用指数、氮吸收效率极显著正相关,与侧根长显著正相关。低氮胁迫下油菜主根长、侧根长和根冠比增加,且根冠比与侧根长显著正相关,说明低氮胁迫下侧根伸长是油菜苗期适应低氮胁迫的重要途径之一。相比氮利用效率,氮吸收效率对油菜苗期生物量的形成影响更大,油菜苗期生物量与氮吸收效率存在极显著相关性,侧根长、氮累积量、氮利用指数与氮吸收效率存在显著正相关。因此,生物量可作为评价油菜苗期氮效率的主要指标。以不同氮水平下植株生物量的平均值为阈值,将162份品系按氮效率分类:双高效型23份、双低效型28份、中间型111份。进一步分析表明,双高效型油菜生物量、氮累积量、氮吸收效率均高于双低效型油菜,低氮胁迫下双高效型油菜在氮吸收累积方面更有优势,表明油菜苗期生物量作为指标评价油菜氮效率结果可靠。
To establish a germplasm evaluation method of rapeseed seedling on nitrogen( N) efficiency,hydroponic assay was performed using 162 rapeseed lines under 2 nitrogen levels( control,9 500μmol/L; LN: low N treatment,237. 5μmol/L) at seedling stage. Biological characteristics,including N efficiency and genotypic differences among different rapeseed lines were investigated in 2015 and 2016. Results showed significant differences between 2 treatments on biomass,main root length,lateral root length,root-shoot ratio,N accumulation,N uptake and use efficiency. Among them,seedling biomass was highly positively correlated with N accumulation,N utilization index,and N uptake efficiency. It was positively correlated with lateral root length. Significant increase in main root length,lateral root length and root to shoot ratio were found in LN-treatment. Additionally,a positive correlation was found between root to shoot ratio and lateral root length under LN. It was suggested that facilitating lateral root growth might be an important way for rapeseed respond to LN stress. Result also showed that N uptake efficiency had greater influence on biomass production than that of N use efficiency. Biomass showed highly significant correlation with N uptake efficiency. Lateral root length,N accumulation and N utilization index also exhibited significant correlation with N uptake efficiency. Therefore,biomass was chosen to be a major indicator for evaluating rapeseed N efficiency at seedling stage. And lateral root length,N accumulation and N utilization index might be assistant indexes. Thus 162 rapeseed lines were further classified into 3 types based on average biomass under 2 N levels. Specifically,23 of them were double-high N efficiency rapeseed genotypes,28 were double-low N efficiency rapeseed genotypes,and 111 were the middle type. Further analysis revealed that biomass,N accumulation and N utilization index of double-high N efficiency genotypes were indeed greater than those of double-low N efficiency genotypes. Moreover,the
作者
顾炽明
韩配配
胡琼
李银水
廖祥生
张志华
谢立华
胡小加
秦璐
廖星
GU Chi-ming;HAN Pei-pei;HU Qiong;LI Yin-shui;LIAO Xiang-sheng;ZHANG Zhi-hua;XIE Li-hua;HU Xiao-jia;QIN Lu;LIAO Xing(Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetics Improvement of Oil Crops,Ministry of Agriculture,Wuhan430062,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2018年第6期851-860,共10页
Chinese Journal of Oil Crop Sciences
基金
国家重点研发计划项目(2016YFD0100202-5)
国家绿肥产业技术体系专项(CARS-22)
中国农业科学院科技创新工程(CAAS-ASTIP-2013-OCRI)
关键词
甘蓝型油菜
苗期
低氮胁迫
氮效率
基因型差异
rapeseed
seedling stage
low nitrogen stress
nitrogen efficiency
genotype difference
