摘要
目的观察柴胡疏肝散对"怒伤肝"大鼠神经内分泌免疫变化及对下丘脑-垂体-肾上腺轴、蓝斑-交感-肾上腺髓质介导的介质反应的调控作用。方法将120只Wistar大鼠随机分成正常对照组、模型组、中药高剂量组、中药中剂量组、中药低剂量组,后4组以通电30s破坏双侧膈区建立"怒伤肝"应激动物模型,中药低、中、高剂量组分别灌胃给予0.3、0.6、1.2g/(kg·d)柴胡疏肝散。结果与模型组比,中药中剂量组大鼠海马γ-氨基丁酸A型受体(GABAA)-α2mRNA表达增多,大脑皮层中孕酮水平升高(P<0.05),大鼠海马去甲肾上腺素(NE)、5-羟色胺(5-HT)、3,4-二羟基苯乙酸(DOPAC)水平降低(P<0.05),脑干组织中NE、5-HT、多巴胺、DOPAC水平降低(P<0.05)。结论柴胡疏肝散可通过多个作用靶点,对"怒伤肝"大鼠多个生理反应环节进行调节。
Objective To observe the effect of Chaihu Shugan powder on neuroendocrine immunity and mediator response mediated by hypothalamus-pituitary-adrenal axis,locus coeruleus-sympathetic-adrenal medulla in rats with"angry liver injury".Methods 120Wistar rats were randomly divided into normal control group,model group,traditional Chinese medicine(TCM)high-dose group,TCM medium-dose group,and TCM low-dose group.After30seconds of electrification,the bilateral diaphragm was destroyed the"angry injury liver"stress model was established.In the stress animal model,the TCM low,medium and high-dose group were given0.3,0.6,1.2g/(kg·d),respectively.Results Compared with the model group,the expression of Gamma-aminobutyric acid type A receptor(GABAA)α2mRNA in the hippocampus and the progesterone content in the cerebral cortex increased(P<0.05),and the contents of Norepinephrine(NE),5-hydroxytryptamine(5-HT)and dihydroxyphenylacetic acid(DOPAC)in the hippocampus of rats decreased(P<0.05).The contents of NE,5-HT,dopamine and DOPAC in brainstem tissues decreased(P<0.05).Conclusion Through multiple targets,Chaihu Shugan powder can regulate many physiological reactions related to"anger liver"rats.
作者
杨军平
王莹
肖亮
YANG Junping;WANG Ying;XIAO Liang(Department of Clinical Laboratory,The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi330006,China)
出处
《国际检验医学杂志》
CAS
2018年第24期3002-3005,共4页
International Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(81860806)
江西省卫计委中医药科技项目(2016A135)
关键词
柴胡疏肝散
怒伤肝
信号传导通路
大鼠
Chaihu Shugan powder
anger liver
signal transduction pathway
rats
