摘要
目的探讨有丝分裂原活化蛋白激酶激酶激酶3(MEKK3)在肾透明细胞癌(CRCC)中的表达及其临床意义。方法采用免疫组织化学SP法检测70例CRCC组织和30例癌旁正常肾组织中MEKK3的表达,采用免疫印迹法检测4例CRCC组织和癌旁正常肾组织中MEKK3蛋白表达。结果 CRCC组织中,MEKK3的表达高于癌旁正常肾组织,MEKK3蛋白高表达的患者预后明显低于低表达者。多因素Cox回归显示,年龄、肿瘤大小、TNM分期、MEKK3表达水平是CRCC预后的独立危险因素。结论 MEKK3在CRCC的发生发展中可能起重要作用,与CRCC的预后密切相关。
Objective To investigate Mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase3(MEKK3)protein expression of clear renal cell carcinoma(CRCC)and its clinical significance.Methods The expression of MEKK3protein was examined by using SP immunohistochemical staining in70cases of CRCC and30cases of adjacent non-cancerous tissues.Western-blot was performed to detect the expression of MEKK3in4cases of CRCC and adjacent non-cancerous tissues.Results MEKK3expression in CRCC was significantly higher than it was in adjacent non-cancerous tissues.The prognosis of patients with MEKK3protein high expression was significantly poor than those with the lower expression.Multivariate Cox regression analysis demonstrated that age,tumor size,TNM stage,and MEKK3expression level were independent risk factors in the prognosis of CRCC.Conclusion MEKK3may play an important role in the development and progression of CRCC.The expression of MEKK3is positively correlated with the prognosis of CRCC.
作者
黄海鹏
付伟金
韦康来
徐小龙
陈文元
HUANG Haipeng;FU Weijin;WEI Kanglai;XU Xiaolong;CHEN Wenyuan(Department of Urology,The Second Affiliated Hospital of Guangxi Medical University,Nanning530001,China;Department of Urology,The First Affiliated Hospital of Guangxi Medical University,Nanning530001,China;Department of Pathology,The Second Affiliated Hospital of Guangxi Medical University,Nanning530001,China)
出处
《福建医科大学学报》
2018年第5期316-319,共4页
Journal of Fujian Medical University
关键词
肾肿瘤/病理学
腺癌
透明细胞
蛋白激酶类
预后
kidney neoplasms/pathology
adenocarcinoma,clear cell
protein kinases
prognosis
