摘要
目的:研究异迷迭香酸苷介导乳腺癌细胞凋亡的作用及机制。方法:体外培养人乳腺癌MDA-MB-231细胞,在培养液中加入不同浓度异迷迭香酸苷(0.1、1、10μmol/L)进行干预。MTT法检测细胞增殖率,AO/EB法观察细胞凋亡情况,Western blotting分析内质网应激通路及凋亡相关蛋白表达。结果:异迷迭香酸苷浓度为0.1、1、10μmol/L时,细胞增殖率分别为(81.56±6.76)%、(69.33±6.40)%、(44.54±7.37)%,较空白对照组[(100.00±7.69)%]降低(P<0.05,P <0.01);细胞凋亡比例分别为(155.67±14.38)%、(183.14±15.18)%、(224.52±17.26)%,较空白对照组[(100.00±16.54)%]增加(P <0.05,P <0.01);GRP78表达量分别为(1.78±0.16)、(1.83±0.27)、(2.52±0.25),较空白对照组(1.00±0.12)增加(P <0.05,P <0.01);ATF4表达量分别为(1.67±0.15)、(1.69±0.08)、(2.21±0.29),较空白对照组(1.00±0.05)增加(P <0.05,P <0.01);CHOP表达量分别为(1.12±0.07)、(1.23±0.07)、(2.06±0.20),较空白对照组(1.00±0.07)增加(P <0.05,P <0.01);p-PERK表达量分别为(1.14±0.07)、(1.18±0.13)、(1.42±0.10),较空白对照组(1.00±0.04)增加(P <0.05);p-eIF2α表达量分别为(1.54±0.02)、(1.65±0.12)、(1.67±0.17),较空白对照组(1.00±0.18)增加(P <0.05,P <0.01);c-caspase3表达量分别为(1.30±0.14)、(1.51±0.22)、(2.01±0.18),较空白对照组(1.00±0.17)增加(P <0.05,P <0.01);Bcl-2/Bax表达量分别为(0.80±0.06)、(0.74±0.10)、(0.42±0.08),较空白对照组(1.00±0.10)降低(P <0.05,P <0.01)。结论:异迷迭香酸苷可诱导乳腺癌MDA-MB-231细胞凋亡,其机制包括上调GRP78/CHOP信号通路和干扰Bcl2/Bax平衡两方面,从而激活内质网应激反应,诱发细胞凋亡。
Objective To study the effect and mechanism of salviaflaside on apoptosis of breast cancer cells.Methods MDA-MB-231cells were cultured and interfered with different concentrations of salviaflaside(0.1μmol/L,1μmol/L,10μmol/L).The cell proliferation rate was detected by MTT assay.AO/EB method was used to observe the cell apoptosis,and Western blotting analysis was used to detect the protein expressions of endoplasmic reticulum stress pathway and apoptosis.Results With the increase of salviaflaside concentration(0.1,1,and10μmol/L),cell proliferation rate[(81.56±6.76)%,(69.33±6.40)%and(44.54±7.37)%]was significantly decreased compared with control group[(100.00±7.69)%,P<0.05,P<0.01].Cell apoptosis rate[(155.67±14.38)%,(183.14±15.18)%and(224.52±17.26)%]was significantly decreased compared with control group[(100.00±16.54)%,P<0.05,P<0.01].GRP78expression[(1.78±0.16),(1.83±0.27)and2.52±0.25)]was significantly decreased compared with control group[(1.00±0.12),(P<0.05,P<0.01)].ATF4expression[(1.67±0.15),(1.69±0.08)and(2.21±0.29)]was significantly decreased compared with control group[(1.00±0.05),(P<0.05,P<0.01)].CHOP expression[(1.12±0.07),(1.23±0.07)and(2.06±0.20)]was significantly decreased compared with control group[(1.00±0.07),(P<0.05,P<0.01)].p-PERK expression[(1.14±0.07),(1.18±0.13)and(1.42±0.10)]was significantly decreased compared with control group[(1.00±0.04),(P<0.05)].p-eIF2αexpression[(1.54±0.02),(1.65±0.12)and(1.67±0.17)]was significantly decreased compared with control group[(1.00±0.18),P<0.05,P<0.01].c-Caspase3expression[(1.30±0.14),(1.51±0.22)and(2.01±0.18)]was significantly increased compared with control group[(1.00±0.17),P<0.05,P<0.01].Bcl-2/Bax expression[(0.80±0.06),(0.74±0.10)and(0.42±0.08)]was significantly increased compared with control group[(1.00±0.10),P<0.05,P<0.01].Conclusion The apoptosis of MDA-MB-231cells can be induced by salviaflaside,the mechanism of which is to interfere with Bcl2/Bax equilibrium by activating the endoplasmic reticulum stress th
作者
吴元肇
曾勇
郑克思
吴林斌
WUYuan-zhao;ZENG Yong;ZHENG Ke-si(Oncological Surgery Department, Wenzhou People' s Hospital ofZhejiang Province, Wenzhou (325035), China)
出处
《中国中西医结合外科杂志》
CAS
2018年第6期749-753,共5页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
