摘要
目的热损伤引起肺水肿,可导致急性呼吸窘迫综合征、癫痫、多器官衰竭甚至死亡。文中采用不同浓度姜黄素预处理干热环境热射病大鼠,探讨其肺组织炎症因子及血清内毒素的变化及相关机制。方法将50只SD大鼠随机数字表法分为5组(n=10):常温对照组,干热对照组,低、中、高浓度姜黄素预处理组(姜黄素50、100、200 mg/kg预处理)。常温对照组、干热对照组给予等渗盐水灌胃,姜黄素预处理组给予不同浓度的姜黄素灌胃,1次/d,连续7 d。第8天除常温对照组外,其余4组大鼠转移至西北特殊环境人工实验舱内进行实验,环境温度(41±0.5)℃,湿度(10±1)%。大鼠在实验的第150分钟达到热射病状态,麻醉后处死,留取血液、肺组织进行分析。观察各组大鼠肺组织病理变化,检测肺组织中炎症因子IL-1β、IL-6、TNF-α以及血浆中LPS表达水平的变化。结果常温对照组,干热对照组,低、中、高浓度姜黄素预处理组IL-1β浓度分别为[(39.50±2.87)、(133.17±3.63)、(127.31±2.12)、(89.57±3.59)、(57.02±5.46) pg/mL],IL-6浓度分别为[(84.76±3.30)、(234.48±3.32)、(224.70±3.13)、(176.82±14.56)、(132.93±4.70) pg/mL],TNF-α浓度分别为[(132.97±8.52)、(238.26±4.46)、(227.98±5.82)、(219. 04±4. 85)、(201. 47±9. 69) pg/mL],内毒素浓度分别为[(0. 80±0. 50)、(17. 19±2. 64)、(10. 30±1.59)、(8.91±1.20)、(7.51±1.20) EU/mL]。与干热对照组比较,常温对照组、低、中、高浓度姜黄素预处理组IL-1β、IL-6、TNF-α和脂多糖浓度差异有统计学意义(P<0.01);与低浓度姜黄素预处理组比较,中、高浓度姜黄素预处理组IL-1β、IL-6、TNF-α和脂多糖浓度降低(P<0.05);与中浓度姜黄素预处理组比较,高浓度姜黄素预处理组脂多糖浓度降低(P<0.01)。Pearson相关分析提示,血浆中脂多糖与肺组织中炎症因子IL-1β、IL-6、TNF-α间呈正相关(相关系数分别为r=0.866,P=0.000; r=0.900,P=0.000; r=0.885,P=0.000
Objective Thermal injury causes pulmonary edema,which may lead to acute respiratory distress syndrome,sepsis,multiorgan failure and even death.The article aimed to study the mechanism of curcumin pretreatment on inflammatory factors in lung tissues and serum endotoxin of rats with dry-heat environment. Methods A total of 50 SD rats were randomly divided into 5 groups(n=10):normal control group,dry heat control group,low concentraion group(50mg/kg curcumin pretreatment group),middle concentraion group(100mg/kg curcumin pretreatment group),and high concentration group(200mg/kg curcumin pretreatment group).Rats in normal control group and dry heat control group were given normal saline by gavage,while rats in 3 curcumin pretreatment groups were given curcumin of different concentrations(50 mg/kg,100 mg/kg,200 mg/kg)by gavage once a day for 7 consecutive days.At 8d,all the other 4 groups except normal control group were transferred to the climate cabin(The Simulated Climate Cabin for Special Environment of Northwest of China)with the condition of(41±0.5)℃,(10±1)%relative humidity.The rats were put in the dry-heat environment for 150min,then they were anaesthetized and sacrificed at 150min to collect the blood,lung tissues for further analysis.Observation was made on the pathological changes of lung tissues of rats in each group and the changes of inflammatory factors such as IL-1β,IL-6,TNF-αand LPS. Results Compared with dry heat control group,the concentrations of IL-1β,IL-6,TNF-αand LPS in normal control group,curcumin pretreatment groups with low concentration,middle concentration and high concentration were significantly higher(P<0.01).The concentrations of IL-1β,IL-6,TNF-αand LPS in curcumin pretreatment group with low concentration were significantly lower than those curcumin pretreatment groups with middle concentration and high concentration(P<0.05).Compared with curcumin pretreatment group with middle concentration,LPS concentration of curcumin pretreatment group with high concentration decreased s
作者
李佳佳
马娜
董翔
曹薇
姜婧
康燕
李建瑛
宋来阳
许琴
刘江伟
LI Jia-jia;MA Na;DONG Xiang;CAO Wei;JIANG Jing;KANG Yan;LI Jian-ying;SONG Lai-yang;XU Qin;LIU Jiang-wei(Key Laboratory of Special Environmental Medicine of Xinjiang,General Hospital of Xinjiang Military Command,Urumqi 830000,Xinjiang,China;Ultrasonography Department,the No.69240 Army Hospital,Urumqi 830000,Xinjiang,China)
出处
《医学研究生学报》
CAS
北大核心
2018年第12期1258-1261,共4页
Journal of Medical Postgraduates
基金
新疆维吾尔自治区自然科学基金项目(2015211C231)
关键词
姜黄素
干热环境
热射病
大鼠
肺损伤
内毒素
炎症因子
curcumin
dry-heat environment
heat stroke
rats
lung injury
lipopolysaccharide
inflammatory
