摘要
目的:建立白及药材的高效液相色谱(HPLC)指纹图谱,并对样品进行聚类。方法:采用HPLC法,色谱柱为Diamonsil C18,流动相为0.1%磷酸溶液-乙腈(梯度洗脱),流速为1.0 mL/min,检测波长为270 nm,柱温为30℃,进样量为10μL。以1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯为参照,绘制10批药材样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004 A版)进行相似度评价,确定共有峰,并采用SPSS 23.0统计软件对其进行聚类分析。结果:10批药材样品的HPLC图谱有15个共有峰,其中9批相似度均大于0.90;经验证,10批药材样品HPLC图谱与对照指纹图谱具有较好的一致性。10批药材样品可聚为3类,S1、S2、S3、S4、S6、S8、S9、S10聚为第1类,S7聚为第2类,S5为第3类。结论:所建HPLC指纹图谱和聚类分析结果可为白及药材的质量控制提供参考。
OBJECTIVE:To establish HPLC fingerprint of Bletilla striata,and conduct cluster analysis.METHODS:HPLC method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consisted of 0.1%phosphoric acid solution-acetonitrile(gradient elution)at the flow rate of 1.0 mL/min.The detection wavelength was set at 270 nm,and column temperature was 30℃.The sample size was 10μL.Using 1,4-two[4-(glucose oxy)benzyl]-2-isobutyl malate as reference,HPLC chromatograms of 10 batches of medicinal samples were determined.Similarity evaluation was conducted by using Similarity Evaluation System for Chromatographic Fingerprint of TCM(2004 A edition).Common peaks were determined,and cluster analysis was also conducted for it by using SPSS 23.0 statistic software.RESULTS:There were 15 common peaks in 10 batches of samples,and the similarities of 9 batches were more than 0.90.After validation,HPLC chromatograms of 10 batches of medicinal material were all in agreement with control fingerprint.The 10 batches of samples could be clustered into 3 categories.S1,S2,S3,S4,S6,S8,S9,S10 were clustered into category 1;S7 was clustered into category 2;S5 was clustered into category 3.CONCLUSIONS:Established fingerprint and the cluster analysis results can provide reference for the quality control of B.striata.
作者
刘刚
丁志山
刘育辰
张永萍
万超
罗成凯
LIU Gang;DING Zhishan;LIU Yuchen;ZHANG Yongping;WAN Chao;LUO Chengkai(College of Pharmacy,Guiyang University of TCM,Guiyang 550025,China;National Engineering Research Center for Miao’s Medicine,Guiyang 550025,China;College of Pharmaceutical Science,Zhejiang University of TCM,Hangzhou 310053,China)
出处
《中国药房》
CAS
北大核心
2018年第22期3050-3053,共4页
China Pharmacy
基金
国家自然科学基金资助项目(No.81673672)
国家公益性行业科研专项经费项目(No.201507002)
贵州省教育厅一流学科项目(No.黔教科研发[2017]85-8)
