摘要
采用提取、萃取及柱层析等方法,从山蒟Piper hancei Maxim甲醇提取物的石油醚和氯仿萃取相中分离到6个脂肪链酰胺类化合物,通过核磁共振、质谱并结合相关文献比对,其结构被分别鉴定为已知化合物chingchengenamide A（C1）、N-异丁基-反-2-反-4-癸二烯酰胺（C2）、假荜拨酰胺A（C3）、荜茇宁（C4）、N-p-香豆酰酪胺（C5）和N-反式-阿魏酰酪胺（C6）,其中C1为首次从山蒟中获得。利用幼虫浸液法测试了各化合物对白纹伊蚊Aedes albopictus和致倦库蚊Culex pipiens quinquefasciatus幼虫的12 h杀虫活性。结果表明：在20 mg/L下,化合物C1对白纹伊蚊的杀虫活性较高,其校正死亡率为100%,LC50值为5.37 mg/L;化合物C1、C2、C3和C4对致倦库蚊的杀虫活性较高,20 mg/L下的校正死亡率分别为100%、88.5%、100%和100%,LC50值分别为1.03、9.68、3.08和2.87 mg/L。
Six aliphatic chain amides,including chingchengenamide A（C1）,N-isobutyldeca-trans-2-trans-4-dienamide（C2）,retrofractamide A（C3）,piperlonguminine（C4）,N-p-coumaroyltyramine（C5）and N-trans-feruloyltyramine（C6）were isolated from petroleum ether and chloroform extraction phase of the methanol extract of the whole plant of Piper hancei.The compound C1 was isolated from P.hancei for the first time.The insecticidal activities of these compounds had been tested by larval immersion method.The bioassay results showed that C1 had significant insecticidal activity with the corrected mortality rate（after 12 h）of 100%at the mass concentrations of 20 mg/L and LC50 value（after 12 h）of 5.37 mg/L against 4^th instar larvae of Aedes albopictus.The compound C1,C2,C3 and C4 also exhibited high activities against 4^th instar larvae of Culex pipiens quinquefasciatus with the corrected mortality rates of 100%,88.5%,100%and 100%,respectively,and LC50 values of 1.03,9.68,3.08 and 2.87 mg/L,respectively.
作者
董存柱
郭锦全
周学明
徐汉虹
DONG Cunzhu;GUO Jinquan;ZHOU Xueming;XU Hanhong(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China;College of Biotechnology,Fujian Vocational College of Agriculture,Fuzhou 350119,China;College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China;College of Agriculture,South China Agricultural University,Guangzhou 510642,China)
出处
《农药学学报》
CAS
CSCD
北大核心
2018年第5期679-683,共5页
Chinese Journal of Pesticide Science
基金
海南省重点研发计划(ZDYF2016184)
海南省自然科学基金面上项目(317046)
国家自然科学基金(31360447)
关键词
山蒟
脂肪链酰胺
植物源杀虫剂
分离鉴定
白纹伊蚊
致倦库蚊
杀虫活性
Piper hancei Maxim
fatty chain amide
botanical insecticide
isolation and identification
Aedes albopictus
Culex pipiens quinquefasciatus
insecticidal activity
