摘要
利用磷酸联合过氧化氢(H_3PO_4-H_2O_2,PHP)预处理玉米芯,并以纤维素酶水解预处理后玉米芯,以酶解效率为指标优化预处理条件。研究结果表明:玉米芯经H_3PO_4-H_2O_2混合液(H_3PO_4质量分数80%)于50℃下预处理4 h后,纤维素质量分数57.38%,纤维素回收率95.84%,半纤维素和木质素的脱除率分别为62.36%和68.97%。在酶用量10 FPIU/g(以葡聚糖质量计)的条件下,72 h酶水解得率为39.12%,相比未经预处理玉米芯的72 h酶水解得率(10.84%)提高了2.61倍。利用红外光谱分析物料预处理后结构的变化,H_3PO_4和H_2O_2在预处理过程中起到了协同作用,能同时去除半纤维素和木质素。相比单独使用H_3PO_4预处理(16.78%)或H_2O_2预处理(20.71%),H_3PO_4-H_2O_2预处理玉米芯的72 h酶水解得率分别提高了133.13%和88.89%。
Corn cob was pretreated by H3PO4-H2O2(PHP)solution and then hydrolyzed by cellulase.The conditions of pretreatment was optimized,which was based on saccharification rate of substrate.Results showed that in pretreated corn cob under the optimized conditions of pretreatment(mass fraction of H3PO4 80%,4 h,50℃),the mass fraction of cellulose was 57.38%;the cellulose recovery,hemicellulose removal and lignin removal were 95.84%,62.36%and 68.97%,respectively.As the dosage of cellulase was 10 FPIU/g glucan,the yield of 72 h enzymatic hydrolysis was 39.12%,which was 2.61 folds higher than that of the enzymatic hydrolysis yield of corn cob without pretreatment(10.84%).Using FT-IR to analyze the change of structure before and after pretreatment,the results showed that,PHP pretreatment had coordinated pretreatment effect in the pretreatment process,which could remove hemicellulose and lignin at the same time.For comparative purpose,H3PO4(16.78%)or H2O2 pretreatment(20.71%)was used independently,to enzymatic hydrolysis of corn cob for 72 h.It was found that the yield of 72 h enzymatic hydrolysis treated by H3PO4-H2O2 pretreatment increased by 133.13%and 88.89%,respectively,while compared with that of H3PO4 or H2O2.
作者
应文俊
吴凯
史正军
杨海艳
郑志锋
杨静
YING Wenjun;WU Kai;SHI Zhengjun;YANG Haiyan;ZHENG Zhifeng;YANG Jing(College of Chemical Engineering,Southwest Forestry University,Kunming 650224,China;University Key Laboratory of Biomass Chemical Refinery&Synthesis;Engineering Laboratory of Biomass High Efficiency Utilization,Southwest Forestry University,Kunming 650224,China)
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2018年第5期100-106,共7页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(31760194)
西南林业大学科技创新基金项目(Z17045)
云南省教育厅科学研究基金项目(2018Y126)。
关键词
条件优化
玉米芯
结构变化
纤维素
酶解
optimization
corn cob
structural changes
cellulose
enzymatic hydrolysis
