摘要
黄曲霉毒素B_1(AFB_1)具有极强的毒性和致癌性,严重危害人和动物健康。通过富集培养、初筛和复筛从土壤中筛选到能降解黄曲霉毒素的菌株,鉴定降解效率最高菌株并研究其降解特征。结果表明,筛选出多株能降解AFB_1菌株,其中菌株A12降解效率最高,通过形态学、生理生化特征及16S rRNA基因系统进化分析鉴定菌株A12为枯草芽孢杆菌,命名为Bacillus subtilis subsp. InaquosorumA12。研究发现枯草芽孢杆菌A12上清液、菌悬液、胞内液能分别降解87.6%、17.3%和10.8%的AFB_1,表明菌株A12分泌至胞外活性物质主导生物降解作用。菌株A12上清液降解AFB_1的最适反应温度为37℃,最适p H为7.0。将该菌接种到AFB_1污染的花生样品,能显著降低AFB_1的含量。为枯草芽孢杆菌A12应用于AFB_1的生物脱毒奠定了基础。
Aflatoxin B1(AFB1)is highly toxic and carcinogenic,which seriously endangers human and animal health.The aflatoxin degradation strains were screened from the soil through enrichment culture,initial screening and rescreening,and the efficient degradation strain was identified and the degradation characteristics were studied.The results showed that the selected strains could degrade AFB1,and the strain A12 was the most efficient one,which was identified as bacillus subtilis,named as Bacillus subtilis subsp.Inaquosorum A12,by morphological,physiological and biochemical analyses and the evolution of 16S rRNA gene system.The degradation rate of supernatant,bacterial suspension,and intracellular fluid of A12 were 87.6%,17.3%and 10.8%AFB1,respectively.The results indicated that degradation activity of strain A12 was located in extracellular fluid,and the optimal reaction temperature and pH value for degradation of AFB1 by supernatant were 37℃and 7.0,respectively.The AFB1 content could be significantly reduced by inoculating strain A12 to peanut sample contaminated by AFB1.This study laid a foundation for the biological detoxification of AFB1 by strain A12.
作者
王明清
于丽娜
张初署
毕洁
孙杰
刘宾
WANG Ming-qing;YU Li-na;ZHANG Chu-shu;BI Jie;SUN Jie;Liu Bin(Shandong Peanut Research Institute,Qingdao Shandong 266100;Management Committee of West Coast Modern Agricultural Demonstration Area,Qingdao Shandong 266000;Agricultural Quality Standards and Testing Technology Institute,Shandong Academy of Agricultural Sciences,Jinan Shandong 250000)
出处
《粮油食品科技》
2018年第5期63-69,共7页
Science and Technology of Cereals,Oils and Foods
基金
山东省自然科学基金(ZR2016CM43
ZR2017MC060
ZR2016YL021
ZR2017MC062)
山东省农业科学院重大科技成果培育计划(2016CGPY10)
山东省农业科学院农业科技创新工程(CXGC2016B17
CXGC2018E21)
山东省2018年度农业重大应用技术创新项目
关键词
黄曲霉毒素B1
筛选
菌株
降解
鉴定
aflatoxin B1
screen
strain
degradation
identification
