摘要
目的:探讨肾母细胞瘤组织中Par-4基因表达量检测及其与肿瘤增殖、侵袭的相关关系。方法:选取确诊肾母细胞瘤的患者60例,活检留取肾母细胞瘤组织、瘤旁组织,采用荧光定量PCR检测其中Par-4基因,增殖相关基因IGF-Ⅱ、IRS-2、TGF-β、PTEN及侵袭相关基因Axin、Ecad、Ki-67表达量的差异;采用Pearson检验评估肾母细胞瘤组织中Par-4基因表达量与增殖、侵袭相关基因表达量的相关关系。结果:肾母细胞瘤组织中Par-4基因表达量低于瘤旁组织;增殖相关基因IGF-Ⅱ、IRS-2、TGF-βmRNA的表达量高于瘤旁组织,PTEN mRNA的表达量低于瘤旁组织;侵袭相关基因Axin、E-cad mRNA的表达量低于瘤旁组织,Ki-67mRNA的表达量高于瘤旁组织。Pearson检验发现,肾母细胞瘤组织中Par-4基因表达量与IGF-Ⅱ、IRS-2、TGF-β、Ki-67表达量呈负相关,与PTEN、Axin、E-cad表达量呈正相关。结论:肾母细胞瘤组织中Par-4基因表达异常降低并且与增殖、侵袭基因表达的改变存在相关性。
Objective:To study the Expression of Par-4 gene in nephroblastoma and its correlation with tumor proliferation and invasion.Methods:60 cases of nephroblastoma patients were chosen as research subject,peritumoral tissue and tumor tissue were obtained and expression of Par-4 gene,proliferation related genes IGF-Ⅱ,IRS-2,TGF-β,PTEN and invasion related genes Axin,E-cad,Ki-67 were detected by fluorescence quantitative PCR.Pearson test was used to analyze the relationship between Par-4 gene and proliferation,invasion related genes.Results:Par-4 in nephroblastoma gene expression was lower than that in in peritumoral tissue;expression of proliferation related gene such as IGF-II,IRS-2,TGF-βmRNA were higher than those in peritumoral tissue,expression of PTEN mRNA was lower than that in peritumoral tissue;expression of invasion related genes such as Axin,E-cad mRNA were lower than those in peritumoral tissue,Ki-67 mRNA was higher than that in peritumoral tissue.Pearson test showed that the expression of Par-4 gene in nephroblastoma tissue was negatively correlated with the expression of IGF-Ⅱ,IRS-2,TGF-β,Ki-67 and positively correlated with the expression of PTEN,Axin,E-cad.Conclusion:Expression of Par-4 gene in nephroblastoma tissue is abnormally decreases and correlates with the change of proliferation and invasive genes.
作者
黄朝友
李响
赖飞
杨振
钱友良
Huang Chaoyou;Li Xiang;Lai Fei(Department of Urology,the Second People's Hospital of Chengdu(Chengdu 610017))
出处
《陕西医学杂志》
CAS
2018年第10期1230-1232,共3页
Shaanxi Medical Journal
