摘要
目的:研究肾母细胞瘤中Par-4的表达及过表达Par-4对肿瘤细胞的杀伤效应。方法:收集肾母细胞瘤组织及肿瘤旁正常组织,测定Par-4的表达量;培养SK-NEP-细胞,转染Par-4质粒后,测定Par-4的表达量、细胞生长情况以及凋亡相关基因的表达量。结果:肾母细胞瘤组织中Par-4的表达量显著低于肿瘤旁正常组织,不同组织分型肿瘤组织中Par-4的表达量无差异,TNM III-IV期、有淋巴结转移肿瘤组织中Par-4的表达量显著低于TNM I-II期、无淋巴结转移的肿瘤组织;转染不同浓度的Par-4质粒后,细胞的生长受到显著抑制,100μg/mL质粒的抑制效应最为显著;转染100μg/mL的Par-4质粒,NF-kB的表达量低于阴性对照组,FasL、Fas、Caspase-3、Caspase-8的表达量高于阴性对照组。结论:肾母细胞瘤中Par-4的表达量显著降低,过表达Par-4能够抑制肿瘤细胞的生长、具有杀伤效应。
Objective： To study the expression of Par-4 in Wilms Tumor and the killing effect of overexpressing Par-4 on tumor cells. Methods： Wilms Tumor tissue and adjacent normal tissue were collected to detect Par-4 expression levels. SK- NEP-1 cells were cultured and transfected with Par-4 plasmid, and then Par-4 expression levels, cell growth and expression levels of apoptosis-related genes were detected, Results： Par-4 expression levels in Wilms Tumor tissue were significantly lower than those in adjacent normal tissue. There was no significant difference in Par-4 expression levels in tumor tissue with different tissue typing, and Par-4 expression levels in tumor tissue with TNM III-IV stage and with lymph node metastasis were significantly lower than those in tumor tissue with TNM I-II stage and without lymph node metastasis. After transfection of different concentrations of Par-4 plasmid, cell growth was significantly inhibited, and the inhibitory effect of 100 μg/mL plasmid was the most significant~ after transfection of 100 μg/mL Par-4 plasmid, NF-kB expression levels were lower than those of negative control group, and expression levels of FasL, Fas, Caspase 3 and Caspase-8 were higher than those of negative control group. Conclusion.. Par-4 expression level significantly decreases in Wilms tumor, and overexpression of Par 4 can inhibit tumor cell growth and has killing effect.
出处
《海南医学院学报》
CAS
2016年第7期639-642,共4页
Journal of Hainan Medical University
基金
福州市科学技术局(2013Y6005)~~
