摘要
目的探讨mi R-26a在溃疡性结肠炎中的表达及其与自噬和凋亡的关系。方法应用实时PCR检测活动期溃疡性结肠炎患者和正常对照者组织及血清中mi R-26a的表达。应用mi R-26a mimics/inhibitor过表达/敲低mi R-26a在Caco-2细胞中的表达,应用流式细胞技术检测凋亡情况,应用Western blotting检测相关蛋白LC3-Ⅱ/LC3-Ⅰ及p62蛋白的表达。结果与正常对照者相比,活动期溃疡性结肠炎患者组织中mi R-26a呈高表达;过表达mi R-26a后Caco-2细胞凋亡增加,自噬水平降低,敲低mi R-26a后Caco-2细胞凋亡减少,自噬增加。结论活动期溃疡性结肠炎组织中mi R-26a高表达,且其高表达促进细胞凋亡并抑制自噬。
Objective To determine miR-26a expression in ulcerative colitis and its relationship with autophagy and apoptosis.Methods miR-26a expression in sera of patients with active ulcerative colitis and normal controls was detected using real-time polymerase chain reaction(PCR).miR-26a mimics/inhibitor were used to upregulate/downregulate miR-26a expression.Apoptosis of Caco-2 cells was detected using flow cytometry.LC3-Ⅱ/LC3-Ⅰand p62 expression was detected by Western blotting.Results miR-26a expression in patients with active ulcerative colitis was higher than in normal controls.Furthermore,miR-26a expression level correlated positively with autophagy and negatively with apoptosis.Conclusion miR-26a is highly expressed in patients with active ulcerative colitis,which promotes apoptosis and reduces autophagy.
作者
曹勇
王东旭
孙妍
杨俊
张营
郑长清
CAO Yong;WANG Dongxu;SUN Yan;YANG Jun;ZHANG Ying;ZHENG Changqing(Department of Gastroenterology,Shengjing Hospital,China Medical University,Shenyang 110004,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2018年第9期807-811,共5页
Journal of China Medical University
基金
辽宁省科学技术计划(2013225303)
关键词
溃疡性结肠炎
自噬
凋亡
ulcerative colitis
autophagy
apoptosis
