摘要
目的:研究溃疡性结肠炎组织中NOX1、NOX2表达量与肠黏膜氧化应激反应、屏障功能损伤的相关性。方法:选择在本院诊断为溃疡性结肠炎的69例患者作为研究的UC组,选择诊断为结肠息肉的78例患者作为研究的息肉组。取溃疡性结肠炎病灶及息肉病灶,检测NOX1、NOX2的表达量以及氧自由基的生成量、凋亡分子和黏膜屏障分子的含量。结果:UC组肠黏膜内NOX1、NOX2的mRNA表达量和蛋白表达量均显著高于息肉组;UC组肠黏膜内LPO、MDA、AOPP、NO、PDCD5、Bax的含量均显著高于息肉组且与NOX1、NOX2的mRNA表达量和蛋白表达量呈正相关,Bcl-2、Cdx1、Cdx2、galectin-1、galectin-3、OCLN、cingulin、ZO-1的含量均显著低于息肉组且与NOX1、NOX2的mRNA表达量和蛋白表达量呈负相关。结论:溃疡性结肠炎组织中NOX1、NOX2的高表达能够激活肠黏膜的氧化应激反应并造成屏障功能损伤。
Objective:To study the correlation of NOX1and NOX2expression in ulcerative colitis tissue with intestinal mucosal oxidative stress response and barrier function injury.Methods:69patients who were diagnosed with ulcerative colitis in Yan'an People's Hospital between May2015and March2017were selected as the UC group of the research,and78patients who were diagnosed with colon polyps were selected as the of polyps group of the research.The ulcerative colitis lesion and polyp lesion were collected to detect the expression of NOX1and NOX2,the generation of oxygen free radicals as well as the contents of apoptosis molecules and mucosal barrier molecules.Results:The mRNA expression and protein expression of NOX1and NOX2in the intestinal mucosa of UC group were significantly higher than those of polyps group;LPO,MDA,AOPP,NO,PDCD5and Bax levels in intestinal mucosa of UC group were significantly higher than those of polyps group and positively correlated with the mRNA expression and protein expression of NOX1and NOX2while Bcl-2,Cdx1,Cdx2,galectin-1,galectin-3,OCLN,cingulin and ZO-1levels were significantly lower than those of polyps group and negatively correlated with the mRNA expression and protein expression of NOX1and NOX2.Conclusion:The high expression of NOX1and NOX2in ulcerative colitis tissue can activate the intestinal mucosal oxidative stress response and result in the intestinal mucosal barrier function injury.
作者
马涛
MA Tao(Department of Gastroenterology, Yan'an People's Hospital, Yan'an, 710061, Shaanxi Province, China)
出处
《海南医学院学报》
CAS
2017年第19期2638-2641,共4页
Journal of Hainan Medical University
基金
陕西省科学技术厅项(9612013Y0286)~~
