摘要
本研究以3个托桂型芍药品种为研究对象,利用高效液相色谱-光电二极管阵列检测(HPLC-DAD)和高效液相色谱-电喷雾离子化-二级质谱联用技术(HPLC-ESI-MS2)鉴定黄色内瓣中黄酮醇及其糖苷类化合物组成,并对其峰面积进行分析。共鉴定出11种黄酮醇及其糖苷类化合物(槲皮素-3,7-O-二葡萄糖苷、山奈酚-3,7-O-二葡萄糖苷、异鼠李素-3,7-O-二葡萄糖苷、槲皮素-3-O-葡萄糖苷-6″-没食子酸酯、槲皮素-3-O-没食子酰葡萄糖苷、山奈酚-3-O-葡萄糖苷-7-O-鼠李糖苷、槲皮素-3-O-β-D-葡萄糖苷、山奈酚-3-O-葡萄糖苷-6″-没食子酸酯、山奈酚-3-O-β-D-葡萄糖苷、异鼠李素-3-O-β-D-葡萄糖苷和山奈酚)。其中,10种成分在3个品种中均被检测出,只有槲皮素-3-O-没食子酰葡萄糖苷在‘向阳奇花’中未检测到,除了山奈酚-3-O-葡萄糖苷-7-O-鼠李糖苷以外,其他成分的峰面积在3个品种黄色内瓣中的差异性较大。本研究为托桂型芍药品种中黄色内瓣的形成机理提供一定的理论依据和黄酮类物质的进一步开发利用等研究奠定基础。
In this study,we identified the composition of flavonols and their glycosides in the yellow inner-petals of three anemone-typed herbaceous peony(Paeonia lactiflora Pall.)cultivars by high-performance liquid chromatography with diode array detector(HPLC-DAD)and by HPLC-electrospray ionization-mass spectrometry(HPLC-ESI-MS 2)and analyzed their relative content.The results showed that 11 flavonols and their glycosides(quercetin-3,7-O-diglucoside,kaempferol-3,7-O-diglucoside,isorhamnetin-3,7-O-diglucoside,quercetin-3-O-glucoside-6"-gallate,quercetin-3-O-galloylglucoside,kaempferol-3-O-glucoside-7-O-rhamnoside,quercetin-3-O-β-D-glucoside,kaempferol-3-O-glucoside-6"-gallate,kaempferol-3-O-β-D-glucoside,isorhamnetin-3-O-β-D-glucoside and kaempferol).10 components of them were detected in three varieties,and only quercetin-3-O-galloylglucoside was not detected in‘Xiangyang Qihua’,but the difference of the relative content of 11 kinds of components in yellow inner-petals of three varieties was larger.This study provides a theoretical basis for the formation mechanism of the yellow inner inner-petals of anemone-typed herbaceous peony cultivars and the further development and utilization of flavonoids.
作者
成梦琳
张艳艳
赵大球
陶俊
CHENG Meng-lin;ZHANG Yan-yan;ZHAO Da-qiu;TAO Jun(College of Horticulture and Plant Protection;Testing center,Yangzhou University,Yangzhou 225009,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第8期1340-1345,共6页
Natural Product Research and Development
基金
江苏省农业三新工程项目(SXGC[2017]297)
关键词
芍药
托桂型
黄色内瓣
黄酮醇
糖苷类化合物
herbaceous peony
anemone-typed
yellow inner-petals
flavonols
glycosides
