摘要
目的:研究文蛤寡肽(Meretrix meretrix oligopeptides,MMO)对高脂饮食诱导小鼠肾损伤的修护作用。方法:建立高脂饲料致小鼠慢性肾损伤模型,通过MMO灌胃30 d后,测定小鼠肾脏指数,检测小鼠血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)浓度,以及小鼠肾组织匀浆中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力和丙二醛(malondialdehyde,MDA)含量,计算小鼠动脉粥样硬化指数(atherosclerosis index,AI),苏木素-伊红(hematoxylin-eosin,HE)染色观察小鼠肾脏病理学改变,免疫组化法测定肾组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、转录生长因子-β1(transforming growth factor-β1,TGF-β1)蛋白表达量。结果:MMO组与模型组比较,小鼠肾脏指数,血清中TG、TC、LDL-C浓度及AI显著下降,HDL-C浓度显著升高,肾组织匀浆中SOD、GSH-Px活力显著升高,MDA含量显著降低,HE染色观察肾组织结构明显好转,免疫组化结果显示MMO可降低肾损伤小鼠α-SMA、TGF-β1蛋白表达水平。结论:MMO对高脂饮食诱导的小鼠肾损伤有较为明显的修护作用。
The protective effect of Meretrix meretrix oligopeptide(MMO)on high fat diet-induced kidney injury in mice was examined.A chronic kidney injury model was established by feeding a high fat diet to mice,and then the mice were lavaged with MMO for 30 days.Kidney index was measured,and serum triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)contents were determined as well as superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)levels in kidney homogenate.In addition,atherosclerosis index(AI)was calculated and renal pathological changes were examined by hematoxylin-eosin(HE)staining.Immunohistochemical staining was used to detect the protein expression ofα-SMA and TGF-β1 in kidney tissue.The results showed that treatment with MMO significantly decreased kidney index,serum TG,TC and LDL-C levels and AI and increased serum HDL-C level as compared to the model group.Treatment with MMO significantly elevated SOD and GSH-Px activities and lowered MDA level in liver homogenate.The immunohistochemical assay showed that MMO markedly down-regulated the protein expression level ofα-SMA and TGF-β1 in kidney tissue.Therefore,MMO can effectively protects against chronic kidney injury induced by high fat diet in mice.
作者
王佳佳
叶蕾
杨最素
余方苗
丁国芳
WANG Jiajia;YE Lei;YANG Zuisu;YU Fangmiao;DING Guofang(Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products,School of Food Science and Pharmaceutics of Zhejiang Ocean University,Zhoushan 316022,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第15期145-151,共7页
Food Science
基金
浙江省自然科学基金项目(LY15C200016
LQ16H300001)
舟山市级公益类科技项目(2015C31012)。
关键词
文蛤寡肽
高脂饲料
肾损伤
修护作用
Meretrix meretrix oligopeptides
high fat diet
kidney injury
protective effect
